Description
Specifications Table
| Product Name | MOPSO Buffer 99.0% For Molecular Biology |
|---|---|
| Pack Size | 25 gm |
| Form | Powder |
| Grade | Molecular Biology |
| Application | DNA/RNA experiments, protein studies, electrophoresis |
Product Description
When precision matters in your molecular biology experiments, the right buffer can make all the difference. MOPSO Buffer 99.0% from eqipped is a high-purity solution designed to maintain stable pH conditions, ensuring accurate results in DNA/RNA research, protein studies, and electrophoresis. Whether you’re a student setting up your first lab experiment or a seasoned researcher refining your protocols, this buffer provides the reliability you need.
MOPSO (3-(N-morpholino)-2-hydroxypropanesulfonic acid) is a zwitterionic buffer known for its excellent pH stability between 6.2 and 7.6, making it a preferred choice for delicate biological applications. Unlike traditional buffers, MOPSO minimizes interference with biological systems, reducing the risk of unwanted side reactions. Its high solubility and low metal-binding properties further enhance its suitability for sensitive assays.
At eqipped, we understand the importance of consistency in lab supplies. That’s why our MOPSO Buffer undergoes rigorous quality checks to meet the 99.0% purity standard, ensuring batch-to-batch reliability. Whether you’re working on PCR, enzyme assays, or cell culture studies, this buffer helps maintain the integrity of your samples.
Upgrade your lab workflow with MOPSO Buffer 99.0%—order now and experience the difference in your research outcomes.
Why MOPSO Buffer is Essential for Molecular Biology
Molecular biology experiments demand buffers that can withstand temperature fluctuations and maintain pH stability. MOPSO Buffer excels in these conditions, making it indispensable for:
1. DNA/RNA Experiments: Prevents pH drift during amplification and sequencing, ensuring accurate nucleotide incorporation.
2. Protein Studies: Ideal for protein purification and crystallization due to its minimal interaction with biomolecules.
3. Electrophoresis: Provides a consistent ionic environment for clear band separation in gels.
Unlike buffers like Tris or phosphate, MOPSO doesn’t chelate metal ions, which is crucial for experiments involving metal-dependent enzymes. Its compatibility with common lab reagents also reduces the need for additional adjustments, saving you time and resources.
How to Use MOPSO Buffer in Your Lab
Preparing a MOPSO buffer solution is straightforward, but precision is key. Here’s a quick guide to get you started:
1. Calculate the Required Concentration: Typically, a 10-50 mM solution works for most applications. For a 50 mM solution, dissolve 1.046 g of MOPSO in 100 mL of water.
2. Adjust the pH: Use a pH meter to titrate the solution to your desired pH (usually between 6.5 and 7.5) with NaOH or HCl.
3. Sterilize if Needed: Filter through a 0.22 µm membrane for sterile applications.
4. Store Properly: Keep the solution at 4°C for short-term use or -20°C for long-term storage.
Always refer to your specific protocol for exact concentrations, as requirements may vary based on the experiment.
Safety and Handling
While MOPSO Buffer is generally safe for lab use, it’s important to follow standard precautions:
Risk Note: Avoid inhalation or direct contact with skin and eyes. Wear appropriate PPE (gloves, lab coat, goggles) when handling. In case of contact, rinse thoroughly with water.
Store the powder in a cool, dry place away from incompatible substances like strong oxidizing agents. For disposal, follow your institution’s chemical waste guidelines.
FAQs
What is the shelf life of MOPSO Buffer 99.0%?
The powder form has a shelf life of 2-3 years when stored properly in a sealed container. Prepared solutions should be used within a few weeks or stored at -20°C for longer stability.
Can MOPSO Buffer be used for cell culture?
Yes, MOPSO is biocompatible and often used in cell culture media to maintain physiological pH, especially in CO₂-free environments.
How does MOPSO compare to MOPS buffer?
MOPSO is a sulfonic acid derivative of MOPS, offering better solubility and lower metal-binding properties, making it more suitable for sensitive biochemical assays.
Is this buffer compatible with PCR?
Absolutely. MOPSO’s pH stability and minimal interference with DNA polymerase make it an excellent choice for PCR buffers.
Where can I buy MOPSO Buffer 99.0% online in India?
You can purchase high-quality MOPSO Buffer 99.0% directly from eqipped, a trusted supplier for lab essentials. Order now for fast delivery across India.
