Specifications Table
Product Name – MOPSO Buffer 99% Extra Pure
Quantity/Pack Size – 50 g
Form – Powder
Grade – Extra Pure (99%)
Application – pH buffering, biochemical research, protein studies
Product Overview
MOPSO Buffer 99% Extra Pure is a high-performance zwitterionic buffering agent designed for precise pH control in laboratory applications. With a purity level of 99%, this extra pure grade ensures minimal interference in sensitive biochemical assays, making it ideal for experiments requiring stable pH conditions. The buffer exhibits a pKa of 6.95 at 20°C, providing optimal buffering capacity in the physiological pH range (6.2–7.6). Its low toxicity and high water solubility (greater than 100 g/L at 20°C) allow for easy preparation of concentrated stock solutions without precipitation. The chemical stability of MOPSO prevents metal ion chelation, reducing the risk of enzyme inhibition or protein denaturation. This buffer is particularly valued for its resistance to enzymatic degradation and compatibility with a wide range of biological systems. Whether used in protein purification, cell culture, or analytical chemistry, MOPSO Buffer 99% Extra Pure delivers consistent performance, ensuring reproducible results across experiments. The powder form allows for flexible concentration adjustments, while the extra pure grade minimizes background contamination, enhancing data accuracy in research applications.
FAQs
1. What is the effective pH range for MOPSO buffer?
MOPSO buffer provides optimal buffering between pH 6.2 and 7.6, with a pKa of 6.95 at 20°C, making it suitable for near-neutral applications.
2. Can MOPSO buffer be used in cell culture experiments?
Yes, MOPSO is biocompatible and non-toxic at typical working concentrations, making it appropriate for cell culture media where stable pH is critical.
3. How does MOPSO compare to HEPES or MOPS buffers?
MOPSO offers a lower pKa than HEPES (7.55) and MOPS (7.2), providing better buffering in the slightly acidic to neutral range without the temperature sensitivity of HEPES.
4. What is the recommended storage condition for MOPSO powder?
Store the powder in a tightly sealed container at room temperature, protected from moisture and light, to maintain stability and prevent degradation.
5. Is this MOPSO buffer compatible with metal-sensitive enzymes?
Unlike some buffers, MOPSO does not chelate metal ions, reducing the risk of enzyme inhibition in metalloenzyme-dependent reactions.