Description
Specifications Table
| Product Name | MOPSO Sodium Salt Buffer extrapure, 99% | 82595 | SRL Chem |
|---|---|
| Pack Size | 50 Gms, 250 Gms, 1 Kg |
| Form | Powder |
| Grade | Extrapure (99%) |
| Application | PCR, electrophoresis, protein purification, cell culture |
Product Description
MOPSO sodium salt buffer is a high-performance biochemical reagent designed for precise pH regulation in laboratory applications. With a purity of 99% (extrapure grade), this buffer ensures consistent results in PCR, gel electrophoresis, and protein-based assays. Its zwitterionic nature provides excellent stability across a wide pH range (6.2–7.6), making it a preferred choice for researchers and diagnosticians.
Whether you’re optimizing DNA amplification or maintaining protein integrity, MOPSO buffer delivers reliable performance. The sodium salt form enhances solubility, allowing for easy preparation of working solutions. For labs prioritizing accuracy, this buffer minimizes pH drift and interference in sensitive experiments.
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Key Applications
MOPSO buffer is widely used in molecular biology and biochemistry due to its non-toxic, non-reactive properties. Common applications include:
- PCR and qPCR assays
- Protein electrophoresis (SDS-PAGE)
- Cell culture media supplementation
- Enzyme activity studies
- DNA/RNA purification protocols
Its compatibility with divalent cations like Mg²⁺ and Ca²⁺ makes it ideal for enzymatic reactions where metal ions are critical.
Why MOPSO Over Other Buffers?
Unlike traditional buffers like Tris or phosphate, MOPSO offers superior pH stability in biological systems. It doesn’t chelate metal ions, ensuring no disruption in enzyme activity. Additionally, its low UV absorbance at 260 nm and 280 nm prevents interference in spectrophotometric assays.
For labs in India, eqipped provides lab-grade MOPSO buffer with batch-to-batch consistency, ensuring reproducible results every time.
Preparation and Storage
To prepare a 1X MOPSO buffer solution, dissolve the powder in deionized water and adjust the pH using NaOH or HCl. Store the powder at room temperature in a dry place, away from direct sunlight. Once reconstituted, the solution can be stored at 4°C for short-term use or aliquoted and frozen for long-term storage.
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Safety and Handling
MOPSO sodium salt is generally non-hazardous but should be handled with standard lab precautions. Wear gloves and safety goggles when preparing solutions. In case of skin or eye contact, rinse thoroughly with water. Dispose of waste according to local regulations.
FAQs
What is the ideal pH range for MOPSO buffer?
MOPSO buffer is most effective between pH 6.2 and 7.6, making it suitable for physiological studies and molecular biology applications.
Can MOPSO buffer be used in cell culture?
Yes, MOPSO is biocompatible and often used in cell culture media to maintain stable pH without toxicity.
How does MOPSO compare to MOPS buffer?
MOPSO has a slightly higher pKa (7.2) compared to MOPS (7.0), offering better buffering capacity in neutral pH ranges.
Is this buffer compatible with PCR?
Absolutely. MOPSO is a common choice for PCR due to its stability and lack of interference with DNA polymerase activity.
Where can I buy MOPSO sodium salt buffer online in India?
You can purchase high-quality MOPSO buffer from eqipped, ensuring fast delivery and lab-grade purity for your research needs.
