Description
Specifications Table
| Product Name | MUG Brilliant Green Bile Broth |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective isolation of E. coli in water, food, and clinical samples |
Product Description
MUG Brilliant Green Bile Broth is a specialized microbiological medium designed for the selective isolation and detection of Escherichia coli in various samples. This broth contains brilliant green dye, which inhibits the growth of Gram-positive bacteria, while bile salts suppress the growth of non-enteric organisms. The inclusion of MUG (4-methylumbelliferyl-β-D-glucuronide) allows for the rapid detection of β-glucuronidase activity, a key characteristic of E. coli.
This medium is widely used in water testing, food microbiology, and clinical diagnostics to confirm the presence of coliforms and E. coli. Its formulation ensures high selectivity and reliability, making it a staple in research labs, educational institutions, and quality control environments.
When preparing the broth, simply dissolve the powder in distilled water, sterilize, and inoculate with the test sample. Incubation at 35-37°C for 24-48 hours allows for clear visualization of E. coli growth, indicated by fluorescence under UV light due to MUG hydrolysis.
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FAQs
What is the shelf life of MUG Brilliant Green Bile Broth?
The powder has a shelf life of 24 months when stored in a cool, dry place. Once prepared, the broth should be used within 7 days if stored at 2-8°C.
Can this broth detect other coliforms besides E. coli?
While it is selective for E. coli, other coliforms may grow but will not produce fluorescence due to the lack of β-glucuronidase activity.
How do I interpret the results?
E. coli presence is confirmed by fluorescence under UV light (366 nm) after incubation. Non-fluorescent growth may indicate other bacteria.
Is this product suitable for student labs?
Yes, it is widely used in educational settings for microbiology experiments due to its reliability and clear results.
Where can I buy MUG Brilliant Green Bile Broth online?
You can purchase authentic Himedia MUG Brilliant Green Bile Broth from eqipped, ensuring quality and fast delivery.
