Description
Specifications Table
| Product Name | MUG Bromocresol Purple Broth with Lactose |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Detection of E. coli in water and food samples |
Product Description
MUG Bromocresol Purple Broth with Lactose is a specialized microbiological medium designed for the detection and enumeration of Escherichia coli in water, food, and environmental samples. This broth contains 4-Methylumbelliferyl-β-D-glucuronide (MUG), which allows for the rapid identification of E. coli through fluorescence under UV light. The inclusion of lactose and bromocresol purple further enhances selectivity by indicating acid production.
This medium is widely used in research labs, educational institutions, and quality control facilities for routine microbial testing. Its high sensitivity and specificity make it a preferred choice for compliance with regulatory standards in food safety and water quality assessments.
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How to Use MUG Bromocresol Purple Broth with Lactose
1. Preparation: Dissolve 500 gm of the powder in 1 liter of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.
2. Inoculation: Aseptically transfer the sample into the sterile broth.
3. Incubation: Incubate at 35-37°C for 24-48 hours.
4. Observation: Check for fluorescence under UV light (366 nm) to confirm E. coli presence.
Applications
This broth is ideal for:
- Water quality testing
- Food safety compliance
- Environmental monitoring
- Educational microbiology labs
Why This Broth Stands Out
Unlike standard media, MUG Bromocresol Purple Broth combines lactose fermentation with MUG fluorescence, offering dual confirmation of E. coli. This reduces false positives and ensures accurate results, making it a cost-effective solution for labs.
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Safety and Storage
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the broth should be used within 24 hours or stored at 2-8°C for short-term use.
FAQs
What is the shelf life of MUG Bromocresol Purple Broth with Lactose?
The powder has a shelf life of 24 months when stored properly. Prepared broth should be used within 24 hours.
Can this broth detect other coliforms?
While it primarily targets E. coli, other lactose-fermenting bacteria may produce acid, indicated by the bromocresol purple color change.
Is this product suitable for student labs?
Yes, it is commonly used in educational settings for microbiology experiments due to its clear results.
How do I interpret the results?
Fluorescence under UV light confirms E. coli. A yellow color indicates acid production from lactose fermentation.
Where can I buy bromocresol purple broth online?
You can purchase authentic MUG Bromocresol Purple Broth with Lactose from eqipped, ensuring quality and fast delivery.
