Description
Specifications Table
| Product Name | MUG EC 0157 Agar, Modified | M1429-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Detection of E. coli O157:H7 in food and water samples |
Product Description
MUG EC 0157 Agar, Modified is a specialized microbiology agar medium formulated for the selective isolation and identification of Escherichia coli O157:H7. This modified agar contains 4-methylumbelliferyl-β-D-glucuronide (MUG), which allows for the detection of β-glucuronidase activity, a key marker for E. coli O157:H7. The medium is widely used in clinical, food, and environmental laboratories to ensure accurate and rapid detection of this pathogenic strain.
Designed for researchers and lab professionals, this agar powder provides consistent results when prepared according to standard protocols. Its high sensitivity and specificity make it an essential tool for microbiological testing, particularly in food safety and water quality assessments. Whether you’re conducting routine diagnostics or advanced research, this agar medium delivers reliable performance every time.
At eqipped, we ensure that all our lab supplies, including this modified agar medium, meet stringent quality standards. Our products are sourced from trusted manufacturers like Himedia, guaranteeing authenticity and precision in your experiments.
Key Applications
This agar medium is primarily used for:
- Detection of E. coli O157:H7 in food samples
- Water quality testing for pathogenic bacteria
- Clinical diagnostics in microbiology labs
- Research studies on bacterial pathogens
Why This Agar Stands Out
Unlike standard agar media, MUG EC 0157 Agar, Modified offers enhanced selectivity and sensitivity for E. coli O157:H7. The inclusion of MUG allows for fluorescent detection under UV light, making it easier to identify positive colonies. This feature is particularly useful in high-throughput screening environments where quick and accurate results are critical.
Additionally, the medium is designed to inhibit the growth of competing flora, ensuring that only the target bacteria are isolated. This reduces false positives and improves the overall reliability of your tests.
Preparation and Usage
To prepare the agar, follow these steps:
- Dissolve 50 grams of the powder in 1 liter of distilled water.
- Heat the solution to boiling while stirring to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C and pour into sterile Petri dishes.
- Allow the agar to solidify before use.
For best results, store the prepared plates at 2-8°C and use within the recommended shelf life. Always follow standard microbiological practices to avoid contamination.
Safety and Handling
While this product is non-hazardous, it is intended for laboratory use only. Always wear appropriate personal protective equipment (PPE) such as gloves and lab coats when handling. Dispose of used media according to your institution’s biohazard waste protocols.
FAQs
What is MUG EC 0157 Agar used for?
It is used for the detection of E. coli O157:H7 in food, water, and clinical samples through β-glucuronidase activity.
How does MUG EC 0157 Agar work?
The agar contains MUG, which fluoresces under UV light when cleaved by β-glucuronidase, indicating the presence of E. coli O157:H7.
Can this agar be used for other bacteria?
While optimized for E. coli O157:H7, it may support the growth of other β-glucuronidase-positive bacteria, but its formulation minimizes false positives.
What is the shelf life of this product?
The powder has a shelf life of 24 months when stored in a cool, dry place. Prepared plates should be used within 4 weeks.
Where can I buy MUG EC 0157 Agar online?
You can purchase this high-quality agar medium from eqipped, a trusted supplier of laboratory reagents and microbiology media.
Need reliable agar for bacterial culture? Buy MUG EC 0157 Agar Modified online today and ensure accurate results in your lab tests.
