Description
Specifications Table
| Product Name | Mycoplasma Broth Base w/o CV (PPLO Broth Base w/o CV) | M267-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Mycoplasma detection, cultivation, and research |
Product Description
Mycoplasma Broth Base w/o CV, also known as PPLO Broth Base, is a specialized medium designed for the isolation and cultivation of mycoplasma organisms. This medium is widely used in microbiology laboratories for diagnostic purposes, research, and quality control in cell culture applications.
Mycoplasma are unique bacteria that lack a cell wall, making them resistant to common antibiotics like penicillin. Detecting mycoplasma contamination is crucial in cell culture work, as it can alter experimental results and compromise research integrity. This broth base provides the necessary nutrients and growth factors to support mycoplasma proliferation, aiding in their detection and study.
Whether you’re a student, researcher, or educator, having a reliable mycoplasma broth medium is essential for accurate and reproducible results. eqipped offers this high-quality medium to ensure your experiments are free from contamination and yield precise outcomes.
Why Use Mycoplasma Broth Base w/o CV?
This medium is specifically formulated to support the growth of fastidious mycoplasma species. It contains essential components like peptides, vitamins, and salts that promote optimal mycoplasma growth without the need for additional supplements. The absence of crystal violet (CV) ensures compatibility with various detection methods, including PCR and ELISA.
For researchers working with cell cultures, regular screening for mycoplasma contamination is a standard practice. Using a dedicated mycoplasma broth medium simplifies the process and enhances detection sensitivity. This product is also suitable for educational purposes, helping students understand mycoplasma biology and detection techniques.
Applications of Mycoplasma Broth Base
Mycoplasma Broth Base w/o CV is versatile and finds applications in multiple areas:
1. Diagnostic Laboratories: Used for detecting mycoplasma infections in clinical samples.
2. Cell Culture Research: Essential for screening cell lines to ensure they are mycoplasma-free.
3. Microbiology Education: Helps students learn about mycoplasma isolation and identification.
4. Quality Control: Employed in pharmaceutical and biotech industries to monitor contamination.
With its broad utility, this medium is a staple in any lab dealing with mycoplasma-related work. eqipped ensures you get a product that meets stringent quality standards, so you can focus on your research without worrying about medium performance.
How to Use Mycoplasma Broth Base w/o CV
Preparing and using this medium is straightforward. Follow these steps for optimal results:
1. Reconstitution: Dissolve the powder in distilled water as per the manufacturer’s instructions. Typically, 21 grams of powder are dissolved in 1 liter of water.
2. Sterilization: Autoclave the medium at 121°C for 15 minutes to ensure sterility.
3. Inoculation: Aseptically inoculate the broth with your sample or cell culture supernatant.
4. Incubation: Incubate at 37°C for 5-7 days, observing for turbidity or color changes indicative of mycoplasma growth.
For best results, always follow the specific protocol provided with the product or as per your lab’s standard operating procedures.
Storage and Handling
Store the powdered medium in a cool, dry place away from direct sunlight. Once reconstituted, the broth should be stored at 2-8°C and used within the recommended timeframe to maintain efficacy.
Handle the medium with care, using appropriate personal protective equipment (PPE) such as gloves and lab coats. While this product is not classified as highly hazardous, good laboratory practices should always be followed to ensure safety.
Risk Note
This product is for laboratory use only. It is not intended for human or animal consumption. Always wear appropriate PPE when handling microbiological media. Dispose of used medium and contaminated materials according to your institution’s biosafety guidelines.
FAQs
What is Mycoplasma Broth Base w/o CV used for?
It is used for the cultivation and detection of mycoplasma organisms in diagnostic, research, and cell culture applications.
Can this medium be used for other bacteria?
While it supports mycoplasma growth, it may not be suitable for other bacterial species due to its specialized formulation.
How long does it take to see mycoplasma growth?
Typically, mycoplasma growth can be observed within 5-7 days of incubation at 37°C.
Is this medium suitable for PCR-based detection?
Yes, the absence of crystal violet makes it compatible with molecular detection methods like PCR.
Where can I buy Mycoplasma Broth Base w/o CV online?
You can purchase this high-quality medium from eqipped, ensuring reliable performance for your lab needs.
