Description
Specifications Table
Product Name – N-Butanol (n-Butyl Alcohol) For Molecular Biology
Quantity/Pack Size – 500 ml
Form – Liquid
Grade – Molecular Biology Grade
Application – DNA/RNA purification, Protein extraction, Solvent for molecular biology techniques
Product Overview
N-Butanol (n-Butyl Alcohol) is a high-purity, essential organic solvent specifically manufactured for critical applications in molecular biology laboratories. This grade of N-Butanol is rigorously tested to ensure the absence of inhibitors that could interfere with sensitive biochemical reactions, making it ideal for DNA and RNA purification protocols, including phenol/chloroform extraction. Its excellent solvent properties also make it valuable in protein precipitation and extraction, where it helps in separating non-polar contaminants. Researchers rely on its consistent quality to achieve reproducible and accurate results in their experiments. Formulated for stability and minimal impurity levels, our N-Butanol is suitable for delicate enzymatic reactions, chromatography, and various other advanced molecular biology techniques. It provides an effective and reliable solution for laboratories demanding a high standard of chemical purity and performance.
FAQs
1. What is the primary application of N-Butanol in molecular biology?
N-Butanol is primarily used in molecular biology for nucleic acid purification, specifically in the phenol/chloroform extraction method to remove residual phenol from aqueous phases containing DNA or RNA. It’s also used in protein extraction and purification.
2. What grade is this N-Butanol and why is it important for molecular biology?
This N-Butanol is Molecular Biology Grade. This specific grade ensures high purity, meaning it’s free from nucleases, proteases, and other contaminants that could degrade or interfere with sensitive biological samples and assays, which is critical for accurate results.
3. How should N-Butanol be stored to maintain its quality?
N-Butanol should be stored in a tightly sealed container in a cool, dry, and well-ventilated area, away from direct sunlight, heat sources, and incompatible materials. Proper storage helps maintain its purity and prevents degradation.
4. Is this N-Butanol suitable for DNA/RNA precipitation?
While N-Butanol is used in the purification steps of DNA/RNA, particularly for phase separation and phenol removal, it is generally not the primary reagent for initiating DNA/RNA precipitation. Ethanol or isopropanol are more commonly used for that purpose.
5. Are there any specific safety precautions when handling N-Butanol?
Yes, N-Butanol is a flammable liquid and can cause irritation to skin, eyes, and respiratory tracts. Always handle it in a well-ventilated area or under a fume hood, wear appropriate personal protective equipment including gloves, eye protection, and a lab coat, and avoid inhalation of vapors.
