Description
Specifications Table
Product Name – N-Methyl-N-(trimethylsilyl)trifluoroacetamide
Quantity/Pack Size – 5GR
Form – Liquid
Grade – 97%
Application – GC-MS derivatization, silylation of alcohols, amines, and carboxylic acids
Product Overview
N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) is a high-purity silylation reagent designed for gas chromatography-mass spectrometry (GC-MS) applications. With a minimum assay of 97%, this reagent ensures reliable derivatization of polar compounds, enhancing volatility and thermal stability for accurate analysis. Its low boiling point (100-102°C) facilitates rapid sample preparation, while the trimethylsilyl (TMS) group effectively replaces active hydrogens in functional groups like alcohols, amines, and carboxylic acids. The reagent is moisture-sensitive, requiring storage under inert conditions to maintain stability. MSTFA is particularly valued for its efficiency in converting non-volatile analytes into volatile TMS derivatives, improving chromatographic separation and mass spectral detection. Its compatibility with automated sample preparation systems makes it a preferred choice for high-throughput laboratories. The 5GR pack size offers sufficient quantity for routine derivatization protocols while minimizing waste.
FAQs
1. What is the shelf life of MSTFA when stored properly?
When stored under nitrogen or argon in a tightly sealed container at 2-8°C, MSTFA maintains its purity for up to 24 months from the date of manufacture. Always check the certificate of analysis for specific batch information.
2. Can MSTFA be used for derivatizing amino acids?
Yes, MSTFA effectively derivatizes amino acids by converting both the amino and carboxyl groups into their trimethylsilyl derivatives, improving their volatility for GC-MS analysis. For optimal results, combine with a catalyst like TMCS (trimethylchlorosilane).
3. What precautions should be taken when handling MSTFA?
MSTFA is moisture-sensitive and should be handled in a dry, inert atmosphere. Use in a fume hood with proper ventilation, as it releases toxic fumes. Avoid skin contact and wear appropriate PPE, including gloves and safety goggles.
4. Is MSTFA compatible with all GC-MS columns?
MSTFA is compatible with most non-polar and mid-polar GC columns, including 5% phenyl-methylpolysiloxane phases. However, highly polar columns may require additional conditioning to prevent silylation of stationary phases.
5. How does MSTFA compare to BSTFA for derivatization?
MSTFA reacts faster than BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) due to its lower molecular weight, making it ideal for quick derivatization protocols. However, BSTFA may offer better derivatization efficiency for sterically hindered compounds.
