Description
Specifications Table
| Product Name | Nile Red (Nile Blue A Oxazone) | 47353 | SRL Chem |
|---|---|
| Quantity/Pack Size | 100 Mg – 1 Gms – 5 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Fluorescence staining of lipids, microscopy, cell biology research |
Product Description
Nile Red is a versatile fluorescent dye widely used in laboratories for staining lipids and hydrophobic molecules. Its unique property of emitting strong fluorescence in lipid-rich environments makes it indispensable for researchers studying cell membranes, lipid droplets, and other biological structures. Whether you’re working on microscopy, flow cytometry, or biochemical assays, this dye provides clear and reliable results.
At eqipped, we ensure you get high-purity Nile Red (Nile Blue A Oxazone) that meets laboratory standards. This product is ideal for students, educators, and researchers who need consistent performance in their experiments. The dye is soluble in organic solvents like DMSO and ethanol, making it easy to prepare working solutions for staining protocols.
If you’re looking to buy Nile Red online, eqipped offers a seamless shopping experience with fast delivery across India. Our packaging ensures the dye remains stable and uncontaminated, so you can trust its performance in your experiments.
Need a reliable supplier for Nile Red? Shop now at eqipped for authentic lab-grade dyes.
How to Use Nile Red in Your Experiments
Using Nile Red effectively requires proper preparation and handling. Here’s a quick guide to help you get started:
1. Preparation: Dissolve Nile Red in a suitable organic solvent like DMSO or ethanol to create a stock solution. A typical concentration ranges from 0.1 to 1 mg/mL, depending on your application.
2. Staining: For cell staining, dilute the stock solution in a buffer or culture medium. Incubate the cells with the dye for 10-30 minutes, then wash to remove excess dye.
3. Visualization: Observe the stained samples under a fluorescence microscope using the appropriate filter set (typically excitation at 488 nm and emission at 520-580 nm).
4. Storage: Store the dye in a cool, dark place to prevent degradation. Always use fresh solutions for best results.
For precise protocols, refer to standard laboratory guidelines or consult your research advisor. If you’re unsure about the right concentration or application, eqipped’s customer support can assist you in selecting the best approach for your needs.
Applications of Nile Red
Nile Red is a multifunctional dye with applications across various fields of research:
Cell Biology: Used to visualize lipid droplets in cells, helping researchers study lipid metabolism and storage diseases.
Microscopy: Enhances the contrast of lipid structures in fluorescence microscopy, making it easier to analyze cellular components.
Biochemistry: Employed in assays to detect and quantify lipids, providing insights into biochemical pathways.
Environmental Studies: Helps in identifying lipid-containing microorganisms in environmental samples.
Whether you’re a student working on a project or a researcher conducting advanced studies, Nile Red is a valuable tool in your lab arsenal. Its versatility and reliability make it a popular choice among scientists.
Safety and Handling
While Nile Red is generally safe for laboratory use, it’s important to follow standard safety protocols:
Risk Note: Nile Red is a chemical dye and should be handled with care. Avoid inhalation, ingestion, or direct contact with skin and eyes. Use appropriate personal protective equipment (PPE) such as gloves and goggles when handling the dye. Work in a well-ventilated area or under a fume hood if necessary. Dispose of waste according to local regulations.
Always refer to the Safety Data Sheet (SDS) provided with the product for detailed handling and disposal instructions. If you have any concerns about safety, eqipped’s team is available to guide you on best practices.
FAQs
What is Nile Red used for in laboratories?
Nile Red is primarily used as a fluorescent dye to stain lipids and hydrophobic molecules in cells and tissues. It’s commonly used in microscopy, flow cytometry, and biochemical assays to visualize and study lipid structures.
How do I prepare a Nile Red solution for staining?
Dissolve Nile Red in an organic solvent like DMSO or ethanol to create a stock solution (0.1-1 mg/mL). For staining, dilute this stock solution in a buffer or culture medium and incubate with your sample for 10-30 minutes before washing and visualization.
Is Nile Red safe to use?
Nile Red is safe for laboratory use when handled properly. Always wear gloves and goggles, work in a well-ventilated area, and follow the safety guidelines provided in the product’s SDS.
Where can I buy Nile Red online in India?
You can buy Nile Red online at eqipped, a trusted supplier of laboratory chemicals and dyes. We offer fast delivery and high-quality products to support your research needs.
What is the shelf life of Nile Red?
When stored properly in a cool, dark place, Nile Red has a long shelf life. However, it’s best to use fresh solutions for staining to ensure optimal fluorescence and accuracy in your experiments.
