Description
Specifications Table
Product Name – N,N-Dimethylformamide, 99.5%, for HPLC
Quantity/Pack Size – 1LT, 2.5LT
Form – Liquid
Grade – HPLC
Application – Solvent for chromatography, sample preparation, and organic synthesis
Product Overview
N,N-Dimethylformamide (DMF), 99.5% purity, is a high-performance solvent specifically formulated for HPLC (High-Performance Liquid Chromatography) applications. This polar aprotic solvent exhibits excellent solvating properties for a wide range of organic and inorganic compounds, making it indispensable in analytical laboratories. Its low volatility and high boiling point (153°C) ensure stability during extended chromatographic runs, while its miscibility with water and most organic solvents enhances versatility in sample preparation. The HPLC-grade purity guarantees minimal UV absorbance and residue, preventing interference in sensitive analytical procedures. DMF’s ability to dissolve both hydrophilic and hydrophobic substances makes it particularly valuable for complex separations. Its chemical structure, featuring a carbonyl group adjacent to a dimethylamino moiety, contributes to its strong dipole moment and solvent power. This grade is rigorously tested for impurities, including water content, which is maintained below 0.05% to meet stringent chromatographic requirements. The solvent’s refractive index (1.430) and density (0.944 g/mL) remain consistent across batches, ensuring reproducible results in quantitative analyses.
FAQs
1. What is the shelf life of HPLC-grade N,N-Dimethylformamide?
When stored properly in tightly sealed original containers at room temperature, away from moisture and direct sunlight, HPLC-grade N,N-Dimethylformamide maintains its specifications for at least 24 months from the date of manufacture. Always check the expiration date on the label before use.
2. Is this DMF compatible with standard HPLC columns?
This HPLC-grade N,N-Dimethylformamide is compatible with most reversed-phase and normal-phase HPLC columns, including C18, C8, and silica-based stationary phases. However, prolonged exposure to high concentrations may affect column lifetime, so it’s recommended to follow manufacturer guidelines for column care.
3. What alternatives can be used if DMF is not suitable for my application?
Depending on your specific requirements, alternatives may include acetonitrile, methanol, or dimethyl sulfoxide (DMSO). Acetonitrile is often preferred for UV detection due to its lower cutoff wavelength, while DMSO offers similar polarity but with different solubility profiles. Always consider the compatibility with your analytes and detection method.
4. How should I handle and store this solvent safely?
Store in a cool, well-ventilated area away from ignition sources and incompatible substances like strong oxidizing agents. Use in a fume hood when possible, as DMF can be absorbed through skin and may cause liver damage with prolonged exposure. Keep containers tightly closed when not in use to prevent moisture absorption.
5. Does this product contain any stabilizers or additives?
This HPLC-grade N,N-Dimethylformamide is supplied without added stabilizers or preservatives to maintain its purity for chromatographic applications. The 99.5% purity specification accounts for trace impurities that are inherent to the manufacturing process and cannot be completely eliminated.
