Specifications Table

Product Name – N,N-METHYLENE BISACRYLAMIDE 99% Molecular Biology

Quantity/Pack Size – 25 g, 100 g

Form – Crystalline Powder

Grade – Molecular Biology, 99% Purity

Application – Cross-linking agent for polyacrylamide gels

Product Overview

N,N-Methylene Bisacrylamide 99% is a high-purity cross-linking reagent specifically designed for molecular biology applications. This crystalline powder ensures superior gel strength and stability in polyacrylamide gel electrophoresis (PAGE), making it ideal for protein and nucleic acid separation. The 99% purity grade minimizes background interference, delivering consistent and reproducible results. Its low moisture content and high solubility in water facilitate easy preparation of gel solutions without clumping or precipitation. The cross-linking efficiency of this compound enhances the mechanical integrity of gels, reducing the risk of cracking or distortion during handling. Compatible with standard buffer systems, it maintains pH stability across a wide range of experimental conditions. Whether used in SDS-PAGE, native PAGE, or DNA sequencing gels, this reagent provides the reliability required for high-resolution separations. The product is rigorously tested for endotoxin and nuclease contamination, ensuring suitability for sensitive downstream applications. Store in a cool, dry place to preserve its reactivity and extend shelf life.

FAQs

1. What is the primary function of N,N-Methylene Bisacrylamide in gel electrophoresis?

It acts as a cross-linker in polyacrylamide gels, forming a three-dimensional network that determines gel pore size and mechanical strength.

2. Is this product compatible with Tris-glycine buffer systems?

Yes, it is fully compatible with standard Tris-glycine and other common electrophoresis buffers without affecting gel performance.

3. How should this reagent be stored to maintain its stability?

Store in an airtight container at room temperature, protected from moisture and light to prevent degradation.

4. Can this be used as a substitute for other bis-acrylamide derivatives?

While chemically similar, substitution may require optimization due to differences in cross-linking efficiency and gel properties.

5. What safety precautions should be taken when handling this powder?

Wear gloves, goggles, and a lab coat to avoid skin and eye contact, and work in a well-ventilated area to minimize inhalation risk.

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