Description
Specifications Table
| Product Name | N,N-Methylene Bisacrylamide (bis-Acrylamide) 3x cryst. extrapure AR, 99.5% |
|---|---|
| Pack Size | 25 Gms – 100 Gms – 500 Gms |
| Form | Crystalline |
| Grade | Extrapure AR |
| Application | Gel electrophoresis, protein analysis, molecular biology |
Product Description
N,N-Methylene Bisacrylamide, commonly known as bis-acrylamide, is a high-purity crosslinking agent essential for polyacrylamide gel electrophoresis (PAGE). This extrapure AR grade product, with 99.5% purity and triple crystallization, ensures consistent and reliable results in protein and nucleic acid separation. Whether you’re a student setting up your first gel or a researcher optimizing protocols, this bis-acrylamide delivers the precision your experiments demand.
At eqipped, we understand the importance of quality reagents in your lab work. That’s why we source this bis-acrylamide from trusted suppliers like SRL Chem, ensuring you get a product that meets international standards. The crystalline form dissolves easily, making it ideal for preparing gels with uniform pore sizes. If you’re looking to buy bis-acrylamide online, this is the go-to choice for both academic and research laboratories.
Upgrade your electrophoresis setup with high-purity bis-acrylamide. Shop now for seamless gel preparation.
For researchers working on SDS-PAGE or native PAGE, the purity of bis-acrylamide directly impacts gel resolution. This extrapure grade minimizes background interference, allowing for sharper band separation. The 3x crystallization process removes impurities that could affect polymerization, giving you gels with consistent mechanical strength. Whether you’re analyzing proteins, DNA, or RNA, this product ensures your data is reproducible and publication-ready.
Students and educators will appreciate how this bis-acrylamide simplifies gel preparation. Its high solubility means no more clumping or incomplete dissolution, saving you time and frustration. The 99.5% purity also means fewer artifacts in your gels, making it easier to teach electrophoresis principles clearly. When you buy bis-acrylamide from eqipped, you’re investing in a product that makes learning and research smoother.
Key Applications
This bis-acrylamide is specifically formulated for:
- Polyacrylamide gel electrophoresis (PAGE) for protein separation
- DNA and RNA analysis in molecular biology
- SDS-PAGE for protein molecular weight determination
- Native PAGE for studying protein complexes
- Graded gel preparation for high-resolution separations
Why This Grade Matters
The extrapure AR grade isn’t just about purity—it’s about reliability. In gel electrophoresis, even minor impurities can lead to:
- Inconsistent polymerization
- Background staining
- Poor band resolution
- Gel degradation over time
With this 99.5% pure bis-acrylamide, you eliminate these variables, ensuring your results are accurate and repeatable. The triple crystallization process removes trace metals and other contaminants that could interfere with your experiments.
Storage and Handling
To maintain the integrity of your bis-acrylamide:
- Store in a cool, dry place away from direct sunlight
- Keep the container tightly sealed to prevent moisture absorption
- Use in a well-ventilated area or under a fume hood
- Avoid skin contact and inhalation of dust
Safety Information
Risk Note: N,N-Methylene Bisacrylamide is a neurotoxin and potential carcinogen. Handle with appropriate personal protective equipment (PPE), including gloves, goggles, and a lab coat. Avoid inhalation and skin contact. Dispose of according to local regulations for hazardous chemicals.
FAQs
What is the difference between bis-acrylamide and acrylamide?
Bis-acrylamide is a crosslinking agent used with acrylamide to form polyacrylamide gels. While acrylamide forms the linear polymer chains, bis-acrylamide creates crosslinks between these chains, giving the gel its structure and mechanical strength.
Can I use this bis-acrylamide for gradient gels?
Yes, this high-purity bis-acrylamide is excellent for gradient gels. Its consistent quality ensures uniform polymerization across different concentrations, which is critical for gradient gel formation.
How do I prepare a 30% acrylamide/bis solution with this product?
For a 30% solution with a 29:1 acrylamide-to-bis ratio, you would mix 29g acrylamide with 1g of this bis-acrylamide and bring to 100ml with water. Adjust ratios based on your specific protocol needs.
What is the shelf life of this bis-acrylamide?
When stored properly in a cool, dry place with the container tightly sealed, this bis-acrylamide has a shelf life of 2-3 years. Always check for any signs of degradation before use.
Is this bis-acrylamide suitable for non-denaturing gels?
Absolutely. This extrapure grade is ideal for both denaturing (SDS-PAGE) and non-denaturing (native PAGE) applications, as it doesn’t introduce contaminants that could affect protein structure or activity.
