Description
Specifications Table
| Product Name | Nutrient Broth No. 2 | 97327 | SRL Chem |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Bacterial growth, microbiology research, diagnostic labs |
Product Description
Nutrient Broth No. 2 is a versatile and reliable medium designed to support the growth of a wide range of non-fastidious microorganisms. Whether you’re a student setting up a basic microbiology experiment or a researcher working on advanced bacterial studies, this nutrient broth powder provides the essential nutrients required for optimal microbial growth.
Formulated with a balanced composition of peptones, beef extract, and sodium chloride, Nutrient Broth No. 2 ensures consistent performance in your lab. It is widely used in educational institutions, research facilities, and diagnostic laboratories for routine culturing, bacterial enumeration, and general microbiological studies.
At eqipped, we understand the importance of quality and reliability in lab supplies. That’s why we offer Nutrient Broth No. 2 from SRL Chem, a trusted name in laboratory reagents. Whether you need a small quantity for a classroom project or bulk supplies for ongoing research, we’ve got you covered.
Buy Nutrient Broth No. 2 online today and ensure your microbiology experiments start with the best foundation.
Understanding Nutrient Broth Composition
The effectiveness of Nutrient Broth No. 2 lies in its carefully balanced formulation. The primary components include:
Peptones provide a rich source of amino acids and nitrogen, essential for bacterial protein synthesis. Beef extract contributes vitamins, minerals, and additional growth factors. Sodium chloride maintains the osmotic balance, creating an environment similar to what many bacteria encounter in nature.
This combination makes Nutrient Broth No. 2 particularly suitable for cultivating common laboratory bacteria like Escherichia coli, Staphylococcus species, and Bacillus species. Its simplicity and effectiveness have made it a staple in microbiology labs worldwide.
Key Applications in Microbiology
Nutrient Broth No. 2 finds applications across various microbiological disciplines:
In educational settings, it serves as the foundation for basic microbiology practicals, helping students understand bacterial growth patterns. Research laboratories use it for preliminary bacterial isolation and as a base for more specialized media. Clinical and diagnostic labs employ it for routine bacterial culturing and sensitivity testing.
Its versatility extends to environmental microbiology, where it helps in the initial isolation of bacteria from soil, water, and other environmental samples. The broth can also be used to prepare agar plates when combined with agar powder, making it a cost-effective solution for labs with varying needs.
Preparing Nutrient Broth No. 2
Preparing this medium is straightforward, making it ideal for both beginners and experienced microbiologists. The general preparation involves dissolving the appropriate amount of powder in distilled water, sterilizing by autoclaving, and then cooling before use.
For most applications, a concentration of 25-30 grams per liter of water is recommended. Always follow the specific instructions provided with your batch of Nutrient Broth No. 2 for optimal results. Proper preparation ensures consistent performance and reliable experimental outcomes.
Storage and Handling
To maintain the quality of your Nutrient Broth No. 2, store the powder in a cool, dry place away from direct sunlight. Once prepared, the liquid medium should be stored at appropriate temperatures and used within the recommended timeframe to prevent contamination.
At eqipped, we ensure that all our lab supplies, including Nutrient Broth No. 2, are packaged and shipped with care to maintain their integrity during transit. Our packaging is designed to protect the product from moisture and other environmental factors that could affect its performance.
Why Nutrient Broth No. 2 is a Lab Essential
In the world of microbiology, having reliable and consistent media is crucial for accurate results. Nutrient Broth No. 2 stands out because of its:
Proven formulation that has been trusted by microbiologists for decades. Versatility in supporting a wide range of bacterial species. Ease of preparation and use, making it suitable for all skill levels. Cost-effectiveness compared to more specialized media.
Whether you’re setting up a new lab or restocking your supplies, including Nutrient Broth No. 2 in your inventory ensures you’re prepared for a wide range of microbiological applications.
Order your Nutrient Broth No. 2 today from eqipped and experience the convenience of quality lab supplies delivered to your doorstep.
FAQs
What is the shelf life of Nutrient Broth No. 2 powder?
The powder typically has a shelf life of 2-3 years when stored properly in a cool, dry place. Always check the expiration date on the package for specific information.
Can Nutrient Broth No. 2 be used for fungal cultures?
While primarily designed for bacterial growth, some fungi may grow in this medium. However, for optimal fungal cultivation, specialized fungal media are recommended.
How do I know if my prepared broth is contaminated?
Signs of contamination include unexpected turbidity, unusual colors, or the presence of unwanted microbial colonies. Always use proper aseptic techniques during preparation and handling.
Is Nutrient Broth No. 2 suitable for anaerobic bacteria?
This broth is generally used for aerobic bacteria. For anaerobic cultivation, additional measures like oxygen removal and specialized media are typically required.
Can I use Nutrient Broth No. 2 for antibiotic sensitivity testing?
While it can be used for basic sensitivity testing, specialized media like Mueller-Hinton broth are often preferred for standardized antibiotic susceptibility testing.
