Description
Specifications Table
| Product Name | o-Nitrophenyl-β-D-Galactopyranoside (ONPG) extrapure, 99% |
|---|---|
| Pack Size | 1 Gms, 5 Gms, 25 Gms |
| Form | Powder |
| Grade | Extrapure (99%) |
| Application | β-galactosidase substrate, molecular biology, enzyme assays |
Product Description
o-Nitrophenyl-β-D-Galactopyranoside (ONPG) is a high-purity substrate used in biochemical assays to detect β-galactosidase activity. This extrapure grade (99%) ensures reliable results in molecular biology research, making it a trusted choice for laboratories and educational institutions. Whether you’re conducting enzyme kinetics studies or bacterial lacZ gene expression analysis, ONPG provides accurate and reproducible data.
At eqipped, we understand the importance of precision in research. That’s why we offer ONPG in multiple pack sizes (1g, 5g, 25g) to suit your experimental needs. Our product undergoes rigorous quality checks to maintain consistency, so you can focus on your work without worrying about reagent variability.
ONPG works by releasing o-nitrophenol, a yellow compound, when hydrolyzed by β-galactosidase. This colorimetric reaction allows for easy quantification, making it indispensable in microbiology, genetics, and biochemistry labs. Researchers and students alike rely on ONPG for its stability and sensitivity in detecting enzyme activity.
Need a reliable ONPG reagent for your next experiment? Shop now at eqipped for fast shipping and competitive pricing.
FAQs
What is ONPG used for in molecular biology?
ONPG is a substrate for β-galactosidase, used to detect enzyme activity in assays like blue-white screening in bacterial cloning.
How do I prepare an ONPG solution for assays?
Dissolve ONPG in a suitable buffer (e.g., Z-buffer) to a concentration of 4 mg/mL. Filter-sterilize if needed.
What’s the difference between ONPG and X-gal?
ONPG produces a yellow color upon hydrolysis, while X-gal produces a blue precipitate. ONPG is used for quantitative assays, whereas X-gal is for qualitative detection.
Can I use ONPG for educational lab experiments?
Yes, ONPG is commonly used in undergraduate biochemistry labs to demonstrate enzyme-substrate interactions.
How should I store ONPG to maintain its purity?
Store ONPG at 2–8°C in a tightly sealed container to prevent moisture absorption and degradation.
