Description
Specifications Table
| Product Name | Oil Red O Solution 0.5% In Isopropanol |
|---|---|
| Quantity/Pack Size | 100ml |
| Form | Liquid Solution |
| Grade | Laboratory Reagent Grade |
| Application | Lipid staining in microscopy, histological studies |
Product Description
Oil Red O Solution 0.5% in Isopropanol is a widely used dye in laboratories for staining lipids, triglycerides, and other neutral fats in tissue samples. This solution is particularly valuable in microscopy and histological studies, providing sharp contrast and clear visualization of lipid droplets. Whether you’re a student, researcher, or educator, this reagent ensures consistent and reliable results for your experiments.
The solution is pre-dissolved in isopropanol, making it ready to use without additional preparation. Its high purity and stability make it ideal for both routine laboratory work and advanced research applications. When you buy Oil Red O solution online from eqipped, you get a product that meets stringent quality standards, ensuring accuracy in your staining procedures.
For best results, follow standard staining protocols. The solution can be used in both fresh and fixed tissue samples, making it versatile for various experimental setups. Its compatibility with common mounting media further enhances its utility in microscopic analysis.
Need a reliable lipid stain for your lab? Buy Oil Red O solution online today and experience precision in your research.
How to Use Oil Red O Solution
Using Oil Red O solution is straightforward, but proper technique ensures optimal staining. Here’s a quick guide:
- Prepare your tissue sections and fix them appropriately.
- Rinse the sections with distilled water to remove any residual fixative.
- Immerse the sections in the Oil Red O solution for the recommended duration (typically 10-15 minutes).
- Wash gently with water to remove excess stain.
- Counterstain if necessary, then mount and observe under a microscope.
Always refer to your specific protocol for exact timings and concentrations, as variations may be required based on the sample type.
Applications of Oil Red O Stain
Oil Red O is a versatile dye with applications across multiple fields:
- Histology: Staining lipid droplets in tissue sections for diagnostic and research purposes.
- Cell Biology: Visualizing lipid accumulation in cell cultures, particularly in studies related to obesity and metabolic disorders.
- Pathology: Identifying fatty changes in liver tissues and other organs.
- Education: A staple in laboratory classes for teaching staining techniques and lipid identification.
Its ability to selectively bind to neutral lipids makes it an indispensable tool in both clinical and research settings.
Safety and Handling
While Oil Red O solution is generally safe when used as directed, it is important to follow standard laboratory safety practices:
- Wear appropriate personal protective equipment (PPE), including gloves and goggles.
- Work in a well-ventilated area or under a fume hood to avoid inhalation of vapors.
- Avoid skin contact and ingestion. In case of accidental exposure, rinse thoroughly with water.
- Store the solution in a cool, dry place away from direct sunlight and heat sources.
For detailed safety information, refer to the Material Safety Data Sheet (MSDS) provided with the product.
Why Oil Red O Solution from eqipped?
When you choose to buy Oil Red O solution online from eqipped, you’re opting for quality and convenience. Our products are sourced from trusted manufacturers like CDH, ensuring that you receive reagents that meet the highest standards of purity and performance. With eqipped, you also benefit from:
- Fast and reliable delivery across India.
- Competitive pricing without compromising on quality.
- Dedicated customer support to assist with your queries.
Whether you’re setting up a new lab or restocking your supplies, eqipped makes it easy to find the right products for your needs.
FAQs
What is Oil Red O solution used for?
Oil Red O solution is primarily used for staining lipids, triglycerides, and other neutral fats in tissue samples. It is commonly employed in microscopy and histological studies to visualize lipid droplets.
How do I prepare Oil Red O solution if I need to make it from powder?
To prepare Oil Red O solution, dissolve 0.5 grams of Oil Red O powder in 100ml of isopropanol. Filter the solution before use to remove any undissolved particles.
Can Oil Red O solution be reused?
It is not recommended to reuse Oil Red O solution, as repeated use can lead to contamination and reduced staining efficiency. Always use fresh solution for best results.
What is the shelf life of Oil Red O solution?
The solution is stable for up to 12 months when stored properly in a cool, dry place away from direct sunlight. Always check the expiration date on the label.
Is Oil Red O solution hazardous?
Oil Red O solution is generally low in toxicity but should be handled with care. Avoid skin contact, inhalation, and ingestion. Use appropriate PPE and follow laboratory safety guidelines.
