Description
Specifications Table
| Product Name | p-Nitrophenylphosphate Disodium Salt Hexahydrate (pNPP) PrimeDx, 99% |
|---|---|
| Pack Size | 250 Gms | 500 Gms |
| Form | Powder |
| Grade | 99% Pure |
| Application | ELISA assays, phosphatase activity detection, biochemical research |
Product Description
When precision matters in your lab, p-Nitrophenylphosphate Disodium Salt Hexahydrate (pNPP) from PrimeDx delivers consistent results. This high-purity substrate is a staple in ELISA assays, offering reliable colorimetric detection for phosphatase activity. Whether you’re a researcher validating biomarkers or an educator demonstrating enzyme kinetics, pNPP ensures clarity in your data.
At eqipped, we understand the need for dependable reagents. That’s why we source pNPP with 99% purity, minimizing background noise in your experiments. The disodium salt hexahydrate form dissolves easily, saving you time during protocol setup. From academic labs to diagnostic centers, this substrate is trusted for its stability and sensitivity.
Upgrade your assays with pNPP substrate—order today for seamless delivery across India.
Why pNPP is Essential for Your Lab
ELISA assays demand substrates that react predictably. pNPP stands out because it produces a bright yellow end product when hydrolyzed by phosphatases, making quantification straightforward. Unlike unstable alternatives, this substrate remains effective even after prolonged storage when handled correctly.
Students and researchers alike appreciate its versatility. Use it to:
- Measure alkaline phosphatase activity in cell lysates
- Validate antibody conjugates in immunoassays
- Teach enzyme kinetics with visible, reproducible results
For educators, pNPP simplifies complex concepts. The vivid color change captures attention, making it easier to explain biochemical principles. And with eqipped’s competitive pricing, you can stock your lab without overspending.
Handling and Storage Guidelines
To maintain pNPP’s integrity, store it in a cool, dry place away from direct sunlight. Always use fresh solutions for accurate results, as repeated freeze-thaw cycles may degrade performance. For long-term storage, keep the powder sealed in its original container.
Need bulk quantities for large-scale projects? eqipped offers flexible pack sizes to match your requirements.
Safety Considerations
While pNPP is generally safe for lab use, follow standard precautions. Wear gloves and goggles when handling the powder to avoid skin or eye irritation. Dispose of waste solutions according to your institution’s chemical safety protocols.
FAQs
What is pNPP used for in ELISA?
pNPP acts as a substrate for alkaline phosphatase, producing a yellow product (p-nitrophenol) that’s measured spectrophotometrically at 405 nm. It’s ideal for quantitative ELISA assays.
How do I prepare a pNPP solution?
Dissolve the powder in a suitable buffer (e.g., diethanolamine or Tris) to a final concentration of 1 mg/mL. Adjust the pH to 9.8 for optimal enzyme activity.
Can I use pNPP for Western blot detection?
Yes, pNPP is compatible with Western blots when using alkaline phosphatase-conjugated secondary antibodies. However, ensure your protocol accounts for its solubility limits.
What’s the shelf life of pNPP substrate?
Unopened, the powder remains stable for 2 years. Once dissolved, use the solution within 24 hours for best results.
Does eqipped offer pNPP in smaller packs for trial use?
Currently, we provide 250 gm and 500 gm packs. For smaller quantities, contact our team to explore custom options.
