Description
Specifications Table
| Product Name | p-Nitrophenylphosphate Disodium Salt Hexahydrate (pNPP) Reagent Grade, 99% |
|---|---|
| Pack Size | 5 Gms, 25 Gms, 250 Gms |
| Form | Powder |
| Grade | Reagent Grade |
| Application | ELISA assays, phosphatase activity detection |
Product Description
p-Nitrophenylphosphate disodium salt hexahydrate, commonly known as pNPP, is a high-purity reagent grade chemical widely used in biochemical and immunological assays. With a purity of 99%, this compound serves as a critical substrate in enzyme-linked immunosorbent assays (ELISA) for detecting alkaline phosphatase activity. When hydrolyzed by phosphatase enzymes, pNPP produces a yellow-colored product that can be quantified spectrophotometrically, making it indispensable in research and diagnostic laboratories.
This reagent grade pNPP is highly soluble in water, ensuring consistent performance in experimental setups. Whether you’re a student conducting academic research or a professional in a diagnostic lab, this product guarantees reliable results. The disodium salt hexahydrate form enhances stability, allowing for long-term storage without compromising quality.
At eqipped, we ensure that every batch of pNPP reagent grade meets stringent quality standards, so you can focus on your experiments with confidence. Whether you need a small quantity for a single experiment or bulk supplies for ongoing research, our flexible pack sizes cater to all requirements.
Upgrade your ELISA assays with high-purity pNPP reagent grade. Order now for precise and reproducible results.
FAQs
What is pNPP used for in ELISA assays?
pNPP is a substrate for alkaline phosphatase. When the enzyme hydrolyzes pNPP, it produces a yellow-colored product that can be measured spectrophotometrically, allowing for quantitative analysis in ELISA assays.
How should I store pNPP reagent grade?
Store pNPP in a cool, dry place in a tightly sealed container. For extended shelf life, keep it in a desiccator to prevent moisture absorption.
Is pNPP reagent grade suitable for educational purposes?
Yes, pNPP is commonly used in academic laboratories for teaching enzyme kinetics, biochemical assays, and ELISA techniques due to its reliability and ease of use.
What safety precautions should I take when handling pNPP?
Wear gloves, safety goggles, and a lab coat. Avoid inhalation and direct contact with skin or eyes. Work in a well-ventilated area or under a fume hood.
Can I buy pNPP reagent grade in bulk?
Yes, eqipped offers pNPP in pack sizes of 5g, 25g, and 250g to accommodate both small-scale and bulk requirements.
