Description
Specifications Table
| Product Name | Peroxidase From Horse Radish~ 250 units/mg | 185195 | CDH |
|---|---|
| Pack Size | 5000 units, 25000 units |
| Form | Lyophilized powder |
| Grade | Research grade |
| Application | ELISA, Western blot, immunohistochemical staining |
Product Description
Peroxidase from horseradish (HRP) is a widely used enzyme in biochemical and diagnostic applications due to its high specificity and stability. This peroxidase enzyme, with an activity of 250 units/mg, is purified to ensure minimal interference in sensitive assays. Whether you’re a student setting up your first ELISA or a researcher optimizing a Western blot protocol, this HRP enzyme offers reliable performance for detecting antigens, antibodies, and other biomolecules.
At eqipped, we understand the importance of consistency in lab supplies. That’s why our peroxidase enzyme undergoes rigorous quality checks to meet international standards. The lyophilized form ensures long-term stability, making it convenient for laboratories with varying usage frequencies. Simply reconstitute with the recommended buffer, and you’re ready to proceed with your experiments.
Need a dependable HRP enzyme supplier in India? Order now and experience seamless delivery with eqipped.
Key Applications
This peroxidase enzyme is versatile and finds use in multiple laboratory techniques:
- ELISA (Enzyme-Linked Immunosorbent Assay): HRP is commonly used as a reporter enzyme to detect target antigens or antibodies through colorimetric or chemiluminescent substrates.
- Western Blotting: Essential for visualizing protein bands after electrophoresis, providing clear and sensitive detection.
- Immunohistochemistry (IHC): Helps in staining tissue sections to identify specific proteins or markers.
- Diagnostic Kits: A critical component in rapid test kits for diseases, hormones, and other biomarkers.
Why This Peroxidase Enzyme Stands Out
Not all peroxidase enzymes are created equal. Here’s what makes this product a preferred choice among researchers and educators:
- High Specific Activity: With 250 units/mg, it ensures strong signal amplification even at low concentrations.
- Low Non-Specific Binding: Purified to reduce background noise in assays.
- Stable and Long Shelf Life: The lyophilized form remains active for extended periods when stored properly.
- Cost-Effective: Available in bulk pack sizes (5000 units and 25000 units) to suit different lab requirements.
Storage and Handling
To maintain the enzyme’s activity, store the lyophilized powder at 2–8°C. Once reconstituted, aliquot and freeze at -20°C for long-term use. Avoid repeated freeze-thaw cycles to preserve enzymatic activity. Always use sterile techniques when handling to prevent contamination.
Safety Information
While peroxidase from horseradish is generally non-toxic, it is intended for laboratory use only. Avoid inhalation or direct contact with skin and eyes. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats when handling. In case of accidental exposure, rinse thoroughly with water and seek medical advice if irritation persists.
FAQs
What is the ideal buffer for reconstituting this peroxidase enzyme?
We recommend using a phosphate-buffered saline (PBS) or Tris-buffer (pH 7.0–7.5) for reconstitution. Avoid buffers containing azide or heavy metals, as they may inhibit enzyme activity.
Can this HRP enzyme be used for both colorimetric and chemiluminescent detection?
Yes, this peroxidase enzyme is compatible with substrates like TMB (for colorimetric assays) and luminol (for chemiluminescent detection), making it versatile for different detection methods.
What is the difference between 5000 units and 25000 units pack sizes?
The pack sizes refer to the total enzymatic activity. The 5000 units pack is ideal for small-scale experiments, while the 25000 units pack offers better value for high-throughput labs or frequent users.
How do I calculate the working concentration for my assay?
The optimal concentration depends on your assay type. For ELISA, a typical range is 1:1000 to 1:5000 dilution. Always perform a titration to determine the best dilution for your specific application.
Does eqipped provide technical support for using this product?
Absolutely! Our team of experts is available to assist with protocol optimization, troubleshooting, and product inquiries. Reach out via our contact page for personalized guidance.
