Description
Specifications Table
Product Name – Phenol
Quantity/Pack Size – 100 g
Form – Crystalline solid
Grade – 99% Pure (Lab Grade)
Application – Organic synthesis, biochemical assays, disinfection, analytical reagent
Product Overview
Phenol, also known as carbolic acid, is a high-purity aromatic organic compound widely recognized for its versatility in laboratory applications. This 99% pure grade ensures minimal impurities, making it suitable for precise chemical reactions, biochemical assays, and analytical procedures. As a crystalline solid with a sharp, medicinal odor, phenol exhibits strong antiseptic and disinfectant properties, which contribute to its role in sterilization protocols. Its molecular structure, consisting of a hydroxyl group attached to a benzene ring, enables high reactivity in electrophilic substitution reactions, making it a cornerstone reagent in organic synthesis. The compound is highly soluble in organic solvents like ethanol and ether but has limited solubility in water, requiring careful handling to avoid crystallization upon exposure to moisture. Due to its corrosive nature, proper storage in airtight containers is essential to maintain stability and prevent degradation. Whether used as a precursor in pharmaceutical synthesis or as a standard in analytical chemistry, this lab-grade phenol delivers consistent performance, ensuring reliable results in demanding research environments. Its low moisture content and high assay purity further enhance its suitability for sensitive applications where contaminant-free reagents are critical.
FAQs
1. What is the shelf life of 99% pure phenol when stored properly?
When stored in a cool, dry place in a tightly sealed container away from direct sunlight and moisture, 99% pure phenol typically retains its stability for up to 24 months from the date of manufacture. Over time, exposure to air or humidity may lead to oxidation or absorption of moisture, which can alter its physical state and purity. For long-term storage, consider dividing the contents into smaller, airtight containers to minimize repeated exposure to ambient conditions.
2. Can this phenol be used directly for DNA or RNA extraction protocols?
While phenol is a common component in nucleic acid extraction buffers, this 99% pure lab-grade phenol is not pre-equilibrated or buffered for direct use in molecular biology applications. For DNA or RNA extraction, it must first be saturated with an appropriate buffer (such as Tris-HCl) and adjusted to a neutral pH to prevent degradation of nucleic acids. Unbuffered phenol can cause hydrolysis of biomolecules, so additional preparation steps are required before use in these protocols.
3. Is this phenol compatible with plastic containers for storage or handling?
Phenol is known to react with certain plastics, particularly polycarbonate and polystyrene, causing leaching or degradation of the container material. For storage, it is recommended to use glass containers with PTFE-lined caps to prevent contamination and ensure chemical stability. During handling, borosilicate glass or HDPE (high-density polyethylene) may be used for short-term contact, but prolonged exposure should be avoided to prevent potential reactions or absorption.
4. What precautions should be taken when dissolving phenol in solvents?
When dissolving phenol in organic solvents like ethanol or ether, always perform the process in a well-ventilated fume hood due to its volatile and toxic fumes. Use gradual addition and gentle stirring to control the exothermic reaction that may occur, especially when mixing with water or aqueous solutions. Wear appropriate personal protective equipment (PPE), including nitrile gloves, safety goggles, and a lab coat, as phenol can cause severe skin burns and respiratory irritation. Avoid using metal spatulas, as phenol can react with certain metals; instead, opt for glass or PTFE-coated tools.
5. How does the purity of this phenol compare to analytical or molecular biology grades?
This 99% pure phenol is classified as lab grade, which is suitable for general chemical synthesis and industrial applications but may contain trace impurities that are not ideal for highly sensitive analytical techniques or molecular biology. Analytical-grade phenol undergoes additional purification steps to achieve higher assay values (often >99.5%) and lower levels of contaminants like heavy metals or oxidizing agents. Molecular biology-grade phenol is further treated to remove nucleases and other enzymes, making it safe for DNA/RNA work. For critical applications, consider upgrading to a higher grade if ultra-pure reagents are required.
