Description
Specifications Table
| Product Name | Phenol Equilibrated with 0.1M Citrate Buffer pH 4.5 for molecular biology w/ Stabilizer | 47484 | SRL Chem |
|---|---|
| Quantity/Pack Size | 100 ml – 500 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | RNA/DNA extraction, nucleic acid purification |
Product Description
When it comes to molecular biology research, the quality of reagents can make or break your experiments. Phenol equilibrated with 0.1M citrate buffer pH 4.5 is a critical component in nucleic acid extraction protocols, ensuring high-purity RNA and DNA isolation. This stabilized phenol buffer is specially formulated to maintain pH stability, preventing degradation of sensitive biological samples during extraction.
Whether you’re a student setting up your first lab experiment or a seasoned researcher optimizing your workflow, this phenol citrate buffer delivers consistent results. The addition of a stabilizer extends shelf life while maintaining performance, making it a reliable choice for long-term lab storage. Unlike generic phenol solutions, this product is pre-equilibrated, saving you time and reducing the risk of pH fluctuations that could compromise your results.
At eqipped, we understand the importance of precision in molecular biology. That’s why we source this phenol buffer from trusted manufacturers like SRL Chem, ensuring you get a product that meets rigorous quality standards. From academic labs to biotech startups, researchers across India trust this stabilized phenol buffer for its reproducibility and efficiency in downstream applications like PCR, sequencing, and cloning.
Upgrade your nucleic acid extraction workflow with this high-performance phenol citrate buffer. Shop now for fast, reliable delivery to your lab.
The buffer’s pH 4.5 formulation is particularly effective for RNA isolation, as it helps partition nucleic acids into the aqueous phase while proteins remain in the organic phase. This selective separation is crucial for obtaining clean, intact RNA samples free from protein contamination. For DNA extraction, the buffer’s properties ensure minimal shearing, preserving the integrity of high-molecular-weight DNA fragments.
Safety is paramount when working with phenol-based reagents. This product includes handling guidelines to ensure safe usage in your lab. Always use appropriate personal protective equipment (PPE) and work in a fume hood when handling phenol solutions. The stabilizer in this formulation not only enhances performance but also contributes to safer storage conditions compared to unstabilized phenol.
For labs looking to streamline their molecular biology workflows, this phenol citrate buffer offers a perfect balance of quality and convenience. No more last-minute pH adjustments or worrying about buffer degradation—just consistent, high-quality results every time. Whether you’re processing a few samples or scaling up for high-throughput applications, this product adapts to your needs.
FAQs
What is the shelf life of this phenol citrate buffer?
The stabilized formulation typically has a shelf life of 12-18 months when stored properly at 2-8°C. Always check the expiration date on the product label before use.
Can I use this buffer for both RNA and DNA extraction?
Yes, this phenol citrate buffer pH 4.5 is suitable for both RNA and DNA extraction protocols. It’s particularly effective for RNA isolation due to the acidic pH.
How should I dispose of used phenol buffer?
Phenol waste should be collected in designated containers and disposed of according to your institution’s hazardous waste protocols. Never pour phenol solutions down the drain.
Is this product compatible with automated nucleic acid extraction systems?
While primarily designed for manual extraction protocols, this buffer can be adapted for some automated systems. Check your equipment’s specifications for compatibility with phenol-based reagents.
What safety precautions should I take when using this product?
Always wear nitrile gloves, safety goggles, and a lab coat. Work in a fume hood and avoid skin contact. In case of spills, follow standard phenol spill protocols immediately.
