Description
Specifications Table
| Product Name | Phenol Red Egg Yolk Polymyxin Agar Base |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Isolation and differentiation of Bacillus species |
Product Description
Phenol Red Egg Yolk Polymyxin Agar Base (M636) is a specialized microbiological medium designed for the selective isolation and differentiation of Bacillus species, particularly Bacillus cereus. This agar base is widely used in clinical and research laboratories to identify and study bacterial colonies based on their enzymatic activity and growth characteristics.
The medium contains phenol red as a pH indicator, which helps in detecting the production of acids or alkalis by the growing bacteria. The addition of egg yolk provides a rich source of lipids and proteins, enhancing the growth of target organisms. Polymyxin B is incorporated to inhibit the growth of Gram-negative bacteria, ensuring selective isolation of Gram-positive Bacillus species.
This agar base is particularly useful in food microbiology, environmental testing, and clinical diagnostics where the presence of Bacillus species needs to be confirmed. The 500 gm pack size is ideal for laboratories with moderate to high sample throughput, ensuring cost-effective and efficient testing.
When preparing the medium, it is essential to follow the manufacturer’s instructions to achieve the correct pH and consistency. The medium should be sterilized by autoclaving and then cooled to the appropriate temperature before pouring into Petri dishes. Once prepared, the agar provides a clear and distinct differentiation of Bacillus colonies, making it easier to interpret results.
For researchers and students working in microbiology, Phenol Red Egg Yolk Polymyxin Agar Base is an indispensable tool. Its reliable performance and ease of use make it a preferred choice for both routine and specialized applications. Whether you are conducting food safety tests or studying bacterial pathogenesis, this medium delivers consistent and accurate results.
If you are looking to buy Phenol Red Egg Yolk Polymyxin Agar Base online, eqipped offers a convenient and reliable platform to purchase high-quality microbiological media. With a focus on providing lab supplies that meet the highest standards, eqipped ensures that you receive products that are both effective and dependable.
FAQs
What is Phenol Red Egg Yolk Polymyxin Agar Base used for?
This agar base is used for the selective isolation and differentiation of Bacillus species, particularly Bacillus cereus, in microbiological testing.
How do I prepare Phenol Red Egg Yolk Polymyxin Agar Base?
Suspend 55 grams of the powder in 1 liter of distilled water, heat to boiling, autoclave at 121°C for 15 minutes, cool to 45-50°C, add sterile egg yolk emulsion, and pour into Petri dishes.
What is the shelf life of Phenol Red Egg Yolk Polymyxin Agar Base?
The powder has a shelf life of 2-3 years when stored in a cool, dry place. Prepared plates should be used within a few weeks when stored at 2-8°C.
Can I use this medium for other bacteria besides Bacillus species?
This medium is specifically formulated for Bacillus species. For other bacteria, consider using a more general-purpose medium or one tailored to the target organism.
Where can I buy Phenol Red Egg Yolk Polymyxin Agar Base online?
You can purchase Phenol Red Egg Yolk Polymyxin Agar Base online from eqipped, a trusted supplier of high-quality lab supplies for students, researchers, and educators.
