Description
Specifications Table
| Product Name | Phenol Red Lactose Broth | M275-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Differential medium for lactose fermentation in microbiology |
Product Description
Phenol Red Lactose Broth is a widely used microbiological medium designed to detect lactose fermentation in bacteria. This medium contains phenol red as a pH indicator, which turns yellow in acidic conditions (positive fermentation) and remains red or turns pink in alkaline conditions (negative fermentation). It is essential for identifying coliforms and other lactose-fermenting bacteria in water, food, and clinical samples.
This 500 gm pack from Himedia (M275) is formulated for consistent performance, ensuring accurate results in research labs, educational institutions, and quality control settings. Whether you’re a student, researcher, or educator, this broth provides a reliable way to study bacterial metabolism and fermentation patterns.
Looking for a trusted supplier of lactose broth for microbiology? eqipped offers high-quality media at competitive prices.
How to Use Phenol Red Lactose Broth
1. Preparation: Dissolve the powder in distilled water as per the manufacturer’s instructions and sterilize by autoclaving.
2. Inoculation: Aseptically inoculate the broth with the test organism.
3. Incubation: Incubate at the appropriate temperature (usually 35-37°C) for 24-48 hours.
4. Observation: Check for color changes. A yellow color indicates lactose fermentation, while red or pink suggests no fermentation.
Applications of Phenol Red Lactose Broth
This medium is commonly used in:
- Microbiology labs for bacterial identification
- Water and food safety testing
- Educational demonstrations of fermentation
- Research on bacterial metabolism
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Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the broth should be used immediately or stored under refrigeration for short-term use. Always follow lab safety protocols when handling microbiological media.
FAQs
What is Phenol Red Lactose Broth used for?
It is used to determine if bacteria can ferment lactose, which helps in identifying coliforms and other lactose-fermenting organisms.
How do I interpret the color changes in the broth?
A yellow color indicates acid production (positive fermentation), while red or pink means no fermentation occurred.
Can I buy lactose broth online in India?
Yes, eqipped offers Phenol Red Lactose Broth (M275) for online purchase with fast delivery across India.
What is the shelf life of this product?
The powder has a long shelf life when stored properly. Check the expiration date on the packaging for exact details.
Is this suitable for educational labs?
Absolutely. This medium is commonly used in universities and colleges for microbiology practicals.
