Description
Specifications Table
| Product Name | Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0 |
|---|---|
| Pack Size | 100 ml, 250 ml, 500 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | RNA/DNA extraction, nucleic acid purification |
Product Description
When it comes to molecular biology research, the quality of your reagents can make or break your experiments. Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0 is a trusted solution for researchers and students working on RNA and DNA extraction protocols. This carefully balanced mixture ensures efficient phase separation, protecting your nucleic acids from degradation while removing proteins and other contaminants.
The pH 8.0 formulation is specifically designed to maintain RNA integrity during extraction, making it ideal for downstream applications like RT-PCR, sequencing, or cloning. Whether you’re isolating plasmid DNA or purifying total RNA from cells, this lab-grade reagent delivers consistent results every time.
At eqipped, we understand the importance of reliable reagents in your lab work. That’s why we source this product from SRL Chem, a brand known for its high-purity molecular biology solutions. The isoamyl alcohol component helps prevent foaming during mixing, while the precise ratio ensures optimal performance in your protocols.
Need a dependable RNA extraction solution? Order your Phenol:Chloroform:Isoamyl Alcohol blend today and experience the difference in your molecular biology workflows.
FAQs
What is the shelf life of Phenol:Chloroform:Isoamyl Alcohol (25:24:1) pH 8.0?
The product typically has a shelf life of 12 months when stored properly at room temperature in a tightly sealed container. However, always check the expiration date on your specific bottle.
Can I use this for both RNA and DNA extraction?
Yes, this formulation is suitable for both RNA and DNA extraction protocols. The pH 8.0 is particularly beneficial for RNA work, but it also works well for DNA isolation.
How should I dispose of waste containing this mixture?
Follow your institution’s guidelines for hazardous chemical waste disposal. Typically, this would involve collecting waste in a designated container and arranging for proper disposal through your lab’s waste management system.
What’s the difference between this and regular phenol chloroform?
The addition of isoamyl alcohol in this 25:24:1 ratio helps prevent emulsification during mixing, leading to cleaner phase separation. The pH 8.0 is also specifically maintained for molecular biology applications.
Can I autoclave this solution?
No, you should not autoclave this mixture. The heat and pressure from autoclaving can alter the chemical composition and affect the performance of the reagent.
