Description
Specifications Table
| Product Name | Phenolphthalein Phosphate HiVeg® Agar |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | HiVeg® (Vegetable-based) |
| Application | Microbiological culture media for selective isolation |
Product Description
Phenolphthalein Phosphate HiVeg® Agar is a specialized microbiological medium formulated for the selective isolation and differentiation of microorganisms. This high-quality agar, manufactured by Himedia, is designed to meet the stringent requirements of research laboratories, educational institutions, and diagnostic centers. The HiVeg® formulation ensures a vegetable-based alternative, making it suitable for applications where animal-derived components are to be avoided.
The medium contains phenolphthalein phosphate, which serves as a substrate for phosphatase enzymes produced by certain bacteria. This enzymatic activity leads to the hydrolysis of phenolphthalein phosphate, resulting in the release of phenolphthalein, which turns pink in alkaline conditions. This color change aids in the identification and differentiation of microorganisms based on their enzymatic profiles.
This agar is particularly useful in clinical and environmental microbiology for the detection of specific bacterial species. Its consistent performance and reliability make it a preferred choice among researchers and lab technicians. Whether you are setting up a new lab or restocking your supplies, eqipped offers this premium agar at competitive prices, ensuring you get the best value for your investment.
Key Applications
Phenolphthalein Phosphate HiVeg® Agar is widely used in various microbiological applications, including:
- Selective isolation of microorganisms in clinical samples
- Differentiation of bacterial species based on phosphatase activity
- Environmental monitoring and water testing
- Educational purposes in microbiology labs
- Research applications requiring high-purity culture media
Its versatility and reliability make it an essential component in any microbiology laboratory. The HiVeg® formulation ensures that the medium is free from animal-derived components, making it suitable for a wide range of applications.
Why This Agar Stands Out
When it comes to selecting the right microbiological medium, quality and consistency are paramount. Phenolphthalein Phosphate HiVeg® Agar from Himedia is known for its exceptional performance and reliability. The medium is designed to provide clear and unambiguous results, making it easier for researchers and lab technicians to interpret their findings.
The HiVeg® formulation is a significant advantage, as it offers a vegetable-based alternative to traditional animal-derived media. This not only makes the medium more ethically sourced but also reduces the risk of contamination and allergic reactions. Additionally, the medium is easy to prepare and use, ensuring that you can focus on your research without worrying about the quality of your culture media.
At eqipped, we understand the importance of having access to high-quality lab supplies. That’s why we offer Phenolphthalein Phosphate HiVeg® Agar at competitive prices, ensuring that you get the best value for your investment. Our commitment to quality and customer satisfaction makes us a trusted partner for all your laboratory needs.
Preparation and Usage
Preparing Phenolphthalein Phosphate HiVeg® Agar is straightforward. Simply dissolve the required amount of powder in distilled water, heat to boiling to ensure complete dissolution, and then autoclave at 121°C for 15 minutes. Once sterilized, allow the medium to cool to approximately 45-50°C before pouring into sterile Petri dishes. The agar will solidify as it cools, providing a stable medium for microbial growth.
When using the agar, it is essential to follow standard microbiological practices to ensure accurate and reliable results. This includes proper handling and storage of the medium, as well as adherence to aseptic techniques during inoculation and incubation. By following these guidelines, you can maximize the effectiveness of the medium and obtain the best possible results.
For optimal performance, store the agar powder in a cool, dry place, away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable time frame to ensure their efficacy.
Safety and Handling
While Phenolphthalein Phosphate HiVeg® Agar is generally safe to handle, it is essential to follow standard laboratory safety protocols. This includes wearing appropriate personal protective equipment (PPE) such as gloves and lab coats, as well as working in a well-ventilated area. Additionally, ensure that the medium is properly disposed of according to your institution’s guidelines for biological waste.
In case of accidental ingestion or contact with eyes or skin, rinse immediately with plenty of water and seek medical advice if necessary. Always refer to the material safety data sheet (MSDS) for detailed information on handling and safety precautions.
FAQs
What is Phenolphthalein Phosphate HiVeg® Agar used for?
Phenolphthalein Phosphate HiVeg® Agar is used for the selective isolation and differentiation of microorganisms based on their phosphatase activity. It is commonly used in clinical and environmental microbiology.
How do I prepare Phenolphthalein Phosphate HiVeg® Agar?
Dissolve the required amount of powder in distilled water, heat to boiling, and autoclave at 121°C for 15 minutes. Cool to 45-50°C before pouring into sterile Petri dishes.
What is the shelf life of Phenolphthalein Phosphate HiVeg® Agar?
The shelf life of the powder is typically 2-3 years when stored in a cool, dry place. Prepared agar plates should be used within a reasonable time frame and stored at 2-8°C.
Is Phenolphthalein Phosphate HiVeg® Agar suitable for vegan applications?
Yes, the HiVeg® formulation is vegetable-based and free from animal-derived components, making it suitable for vegan applications.
Where can I buy Phenolphthalein Phosphate HiVeg® Agar online?
You can buy Phenolphthalein Phosphate HiVeg® Agar online at eqipped. We offer competitive prices and fast delivery across India.