Description
Specifications Table
| Product Name | Phenyl Blood Agar Plate w/ 5% Sheep Blood |
|---|---|
| Quantity/Pack Size | 50 plates |
| Form | Pre-poured agar plates |
| Grade | Laboratory grade |
| Application | Bacterial culture, hemolysis testing, microbiology research |
Product Description
Phenyl Blood Agar Plate with 5% Sheep Blood | MP540-50PT | Himedia is a ready-to-use enriched microbiological culture medium designed for the cultivation and differentiation of a wide range of microorganisms, particularly bacteria that require enriched growth conditions. The medium contains 5% sheep blood, providing additional nutrients and growth factors that support the recovery of both non-fastidious and fastidious organisms in routine microbiological applications.
Blood agar is widely used as a general-purpose enriched medium and is particularly valuable for observing characteristic colony morphology and hemolytic reactions. The addition of sheep blood provides a suitable environment for microbial growth while allowing laboratories to examine changes in the blood surrounding individual colonies. Depending on the organism, colonies may demonstrate different hemolysis patterns, which can provide useful preliminary information during microbial identification.
The Phenyl Blood Agar formulation is designed for microbiological applications where an enriched blood-containing medium is required. The phenyl component of the formulation provides specific characteristics that can support the intended laboratory testing procedure. As with other differential culture media, observations obtained from the plate should be interpreted together with appropriate biochemical, microscopic, molecular, or other confirmatory tests when definitive identification is required.
The MP540-50PT product is supplied as a convenient ready-to-use plate, eliminating the need for laboratories to prepare the agar base, sterilize the medium, add blood, and pour individual plates. This can help streamline laboratory workflows while promoting consistency between tests. The prepared format is especially useful for laboratories that perform regular microbiological culture and screening activities.
Phenyl Blood Agar Plates may be suitable for applications in clinical microbiology, research laboratories, educational institutions, pharmaceutical microbiology, food testing, and other laboratory environments where enriched blood agar is required. The medium can support the examination of colony characteristics and hemolytic reactions, making it useful as part of a broader microbial isolation and identification workflow.
The presence of 5% sheep blood provides a standardized blood supplement for the culture medium. Sheep blood is commonly used in microbiological media because it can support the growth of a variety of organisms while providing a clear background for observing hemolysis. The appearance of alpha, beta, or gamma hemolytic reactions may assist with preliminary differentiation, depending on the microorganism and testing conditions.
For dependable results, plates should be stored, handled, inoculated, incubated, and interpreted according to the manufacturer’s instructions and the laboratory’s validated testing procedures. Appropriate controls should be included wherever required. Suspected organisms should not be identified solely on the basis of colony appearance or hemolysis and should undergo suitable confirmatory testing when necessary.
Overall, the Himedia Phenyl Blood Agar Plate with 5% Sheep Blood provides a convenient prepared medium for enriched microbial cultivation, colony observation, and preliminary differentiation based on growth characteristics and hemolytic reactions.
FAQs
1. What is Phenyl Blood Agar Plate used for?
Phenyl Blood Agar Plates are used as enriched culture media for the cultivation and preliminary differentiation of microorganisms. They can support microbial growth and allow observation of colony characteristics and hemolytic reactions.
2. What is the purpose of adding 5% sheep blood?
Sheep blood enriches the medium with nutrients and growth factors that support the cultivation of various microorganisms. It also allows laboratories to observe hemolytic reactions around microbial colonies.
3. What types of hemolysis can be observed on blood agar?
Microorganisms may produce different hemolytic patterns, commonly described as alpha, beta, or gamma hemolysis. These reactions can provide useful preliminary information during microbial differentiation but may require additional testing for confirmation.
4. Are MP540-50PT plates ready to use?
Yes. The MP540-50PT product is supplied as a prepared agar plate, reducing the need for in-house preparation, sterilization, blood addition, and plate pouring before microbiological testing.
5. What is the pack size and product code?
The product code is MP540-50PT, and the product is supplied as a pack of 50 ready-to-use plates manufactured by Himedia.
