Description
Specifications Table
| Product Name | Phenylalanine Malonate Broth (Shaw and Clarke) |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Phenylalanine deaminase test, Malonate utilization test |
Product Description
Phenylalanine Malonate Broth, also known as Shaw and Clarke medium, is a differential microbiological medium designed for two key tests: the phenylalanine deaminase test and the malonate utilization test. This medium is widely used in clinical and research laboratories to identify and differentiate bacterial species based on their metabolic capabilities.
The phenylalanine deaminase test helps detect the presence of the enzyme phenylalanine deaminase, which converts phenylalanine to phenylpyruvic acid. This reaction is crucial for identifying certain bacterial strains, particularly those in the Proteus and Morganella genera. The malonate utilization test, on the other hand, assesses the ability of bacteria to use malonate as a sole carbon source, aiding in the differentiation of Enterobacteriaceae members.
At eqipped, we ensure that our Phenylalanine Malonate Broth meets the highest quality standards, providing reliable and consistent results for your microbiology experiments. Whether you’re a student, researcher, or educator, this medium is an essential tool for accurate bacterial identification.
Need high-quality microbiology media for your lab? Buy Phenylalanine Malonate Broth online today for precise and dependable results.
FAQs
What is Phenylalanine Malonate Broth used for?
Phenylalanine Malonate Broth is used for the phenylalanine deaminase test and the malonate utilization test, which help identify and differentiate bacterial species based on their metabolic capabilities.
How do I prepare Phenylalanine Malonate Broth?
Dissolve the required amount of powder in distilled water, sterilize by autoclaving at 121°C for 15 minutes, and then inoculate with the bacterial culture you wish to test.
What does a positive result look like in the phenylalanine deaminase test?
A positive result is indicated by a green coloration when a few drops of 10% ferric chloride solution are added to the medium, due to the presence of phenylpyruvic acid.
How long should I incubate the medium?
The medium should be incubated at 35-37°C for 24-48 hours, depending on the bacterial species being tested.
Can I use Phenylalanine Malonate Broth for educational purposes?
Yes, this medium is commonly used in academic settings to teach students about bacterial metabolism and identification techniques.
