Description
Specifications Table
| Product Name | PIPES Buffer extrapure, 99% |
|---|---|
| Pack Size | 25 Gms, 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Extrapure |
| Application | Electrophoresis, cell culture, biochemical assays |
Product Description
When your experiments demand precision, PIPES Buffer extrapure, 99% delivers unmatched stability for critical lab applications. This high-purity buffer powder is specifically formulated to maintain a consistent pH of 6.8, making it indispensable for electrophoresis, cell culture media, and enzymatic assays. Unlike generic buffers, this extrapure grade ensures minimal interference with sensitive biological systems, guaranteeing reliable results every time.
Researchers and students alike trust PIPES buffer for its exceptional performance in maintaining cellular integrity during experiments. Its zwitterionic nature provides superior buffering capacity between pH 6.1–7.5, a range often required for protein studies and molecular biology protocols. Whether you’re running SDS-PAGE gels or culturing mammalian cells, this buffer’s low metal ion content prevents unwanted reactions that could compromise your data.
At eqipped, we understand that lab supplies aren’t just products—they’re the foundation of your discoveries. That’s why we source only the highest-grade chemicals, like this SRL Chem PIPES buffer, to support your research with confidence. Need a buffer that won’t let you down? Order your PIPES Buffer extrapure today and experience the difference purity makes.
FAQs
What is the ideal pH range for PIPES buffer?
PIPES buffer is most effective between pH 6.1–7.5, with optimal buffering capacity around pH 6.8. This makes it particularly suitable for biological systems requiring slightly acidic to neutral conditions.
Can I use PIPES buffer for DNA electrophoresis?
While PIPES can be used for protein electrophoresis, it’s not typically recommended for DNA electrophoresis. For DNA applications, buffers like TAE or TBE are more commonly used due to their compatibility with nucleic acids.
How do I prepare a 1M PIPES buffer solution?
To prepare a 1M solution, dissolve 302.37g of PIPES powder in 800ml of distilled water. Adjust the pH to 6.8 using NaOH or KOH, then bring the volume to 1L with additional distilled water. Sterilize by filtration if needed for cell culture applications.
Is this PIPES buffer suitable for cell culture media?
Yes, this extrapure grade PIPES buffer is excellent for cell culture applications. Its high purity ensures minimal cytotoxicity, and it’s often used in CO₂-independent media formulations to maintain stable pH conditions.
What’s the shelf life of PIPES buffer powder?
When stored properly in a cool, dry place with the container tightly sealed, PIPES buffer powder has a shelf life of at least 2 years. Always check for signs of moisture absorption or clumping before use.
