Description
Specifications Table
Product Name – PIPES Ultrapure Bioreagent
Quantity/Pack Size – 100 g
Form – Powder
Grade – Bioreagent
Application – Buffering agent in cell culture, protein purification
Product Overview
PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)) is a zwitterionic buffer widely recognized for its exceptional pH stability in biological systems. This ultrapure bioreagent grade ensures minimal interference in sensitive assays, with a purity of ≥99% and negligible levels of heavy metals, DNase, RNase, and protease contaminants. The buffer maintains optimal performance between pH 6.1–7.5, making it particularly suitable for applications requiring precise pH control, such as enzyme reactions, cell culture media formulation, and protein crystallization. Its low UV absorbance and non-toxicity further enhance compatibility with spectroscopic techniques and live-cell experiments. The powder form allows for flexible preparation of concentrated stock solutions, while the bioreagent certification guarantees consistency across batches. Unlike conventional buffers, PIPES exhibits minimal temperature-dependent pH shifts, ensuring reliable results in temperature-sensitive protocols. The product is rigorously tested for endotoxin levels (<0.1 EU/mg), making it ideal for demanding research environments where sample integrity is critical.
FAQs
1. What is the recommended working concentration for PIPES buffer?
Typical working concentrations range from 10–50 mM, depending on the application. For cell culture, 20 mM is commonly used.
2. Is this PIPES buffer compatible with divalent cations like Mg²⁺ or Ca²⁺?
Yes, PIPES forms stable complexes with divalent cations, but high concentrations (>10 mM) may require pH readjustment after addition.
3. How should unused PIPES powder be stored for long-term stability?
Store the unopened container at room temperature (15–25°C) in a desiccated environment. Avoid humidity to prevent clumping.
4. What alternatives exist if PIPES interferes with my assay’s fluorescence detection?
Consider MOPS or HEPES buffers, which have lower UV absorbance but different pH ranges (MOPS: 6.5–7.9; HEPES: 6.8–8.2).
5. Does this product contain any animal-derived components?
No, this PIPES buffer is synthetically derived and free from animal-origin materials, suitable for vegan research protocols.
