Description
Specifications Table
Product Name – Polyethyleneimine, branched, M.W. 10,000, 99%
Quantity/Pack Size – 25g, 100g, 500g
Form – Solid (powder/flakes)
Grade – 99%
Application – Transfection reagent, polymer synthesis, surface functionalization
Product Overview
Polyethyleneimine (PEI), branched, with a molecular weight of 10,000 and 99% purity, is a highly efficient cationic polymer widely used in biochemical and materials research. Its branched structure provides high charge density, making it exceptionally effective for nucleic acid condensation and transfection applications. The polymer’s proton sponge effect enhances endosomal escape, significantly improving transfection efficiency in cell culture systems. This grade of PEI is synthesized under controlled conditions to ensure minimal batch-to-batch variation, offering consistent performance in critical applications like gene delivery, protein immobilization, and nanoparticle synthesis. The solid form (powder or flakes) ensures easy handling and long-term stability when stored under recommended conditions. Its compatibility with aqueous solutions allows seamless integration into existing protocols without requiring extensive optimization. The 99% purity level minimizes impurities that could interfere with sensitive biological assays or material properties. Researchers rely on this PEI variant for its reproducibility, scalability, and versatility across diverse experimental setups, from fundamental studies to applied nanotechnology.
FAQs
1. What is the ideal pH range for dissolving branched PEI (MW 10,000)?
Branched PEI dissolves best in slightly acidic to neutral pH (5.0–7.5). Avoid highly alkaline conditions to prevent degradation.
2. Can this PEI grade be autoclaved for sterile applications?
Autoclaving is not recommended as it may cause polymer degradation. Use sterile filtration (0.22 µm) for aseptic preparations.
3. How does branched PEI compare to linear PEI for transfection?
Branched PEI generally offers higher transfection efficiency due to its compact structure and greater proton buffering capacity.
4. What solvents are compatible with this PEI for non-aqueous applications?
It is soluble in polar organic solvents like methanol, ethanol, and DMSO, but solubility varies with concentration.
5. What is the shelf life of unopened PEI under standard storage?
When stored desiccated at 2–8°C, unopened PEI retains stability for at least 24 months.
