Description
Specifications Table
| Product Name | Polyvinylpolypyrrolidone (PVPP) (Crospovidone) Plant Culture Tested | PCT2003 | CDH |
|---|---|
| Pack Size | 25g, 100g, 500g |
| Form | Powder |
| Grade | Plant Culture Tested |
| Application | Polyphenol removal in plant tissue culture, lab research |
Product Description
Polyvinylpolypyrrolidone (PVPP), commonly known as Crospovidone, is a highly effective insoluble polymer used in plant tissue culture and laboratory research. This plant culture-tested grade PVPP is specifically designed to bind and remove polyphenols, tannins, and other inhibitory compounds from plant extracts, ensuring cleaner and more reliable experimental results.
Whether you’re a student, researcher, or educator working in plant biotechnology, this PVPP grade is an essential addition to your lab supplies. Its high purity and consistency make it ideal for applications where precision matters, such as media preparation, DNA/RNA extraction, and enzyme assays.
At eqipped, we understand the importance of quality reagents in scientific work. That’s why we source PVPP from trusted suppliers to ensure you get the best performance in your experiments.
Upgrade your plant tissue culture workflow with high-quality PVPP. Buy online today for fast delivery across India.
Why PVPP is Essential for Plant Tissue Culture
Polyphenols and tannins present in plant tissues can interfere with enzymatic reactions, DNA extraction, and cell culture growth. PVPP acts as an adsorbent, selectively binding these compounds and preventing their negative effects. This makes it indispensable in:
- Plant DNA/RNA isolation protocols
- Protein purification from plant sources
- Media preparation for tissue culture
- Removing browning in plant extracts
Unlike soluble PVP (Polyvinylpyrrolidone), PVPP is insoluble and can be easily removed by centrifugation or filtration, leaving behind a cleaner sample for downstream applications.
Applications in Research and Education
From university labs to advanced research facilities, PVPP is widely used in:
- Molecular biology labs for nucleic acid extraction
- Plant physiology studies
- Phytochemistry research
- Educational demonstrations in biotechnology courses
Its versatility and reliability make it a staple in both academic and industrial settings.
How to Use PVPP in Your Experiments
For optimal results, follow these general guidelines:
- Add PVPP directly to your plant extract or grinding buffer (typically 1-5% w/v).
- Incubate with gentle mixing to allow polyphenol binding.
- Remove PVPP by centrifugation or filtration before proceeding with your protocol.
Always refer to your specific protocol for exact concentrations and incubation times.
Safety and Handling
While PVPP is generally considered non-hazardous, standard lab safety practices should be followed:
- Wear appropriate PPE (gloves, lab coat, safety goggles).
- Avoid inhalation of powder.
- Store in a cool, dry place away from direct sunlight.
FAQs
What is the difference between PVPP and PVP?
PVPP (Polyvinylpolypyrrolidone) is an insoluble cross-linked polymer, while PVP (Polyvinylpyrrolidone) is soluble. PVPP is preferred for polyphenol removal as it can be easily separated from samples.
Can PVPP be autoclaved?
Yes, PVPP can be autoclaved at 121°C for 15-20 minutes without losing its adsorption properties.
What is the typical concentration of PVPP used in plant tissue culture?
Most protocols use 1-5% (w/v) PVPP, but the exact amount depends on the plant species and polyphenol content.
Is this PVPP suitable for food or pharmaceutical applications?
This product is specifically tested for plant culture use and is not intended for food, drug, or cosmetic applications.
How should I store PVPP?
Store in a tightly sealed container at room temperature. Keep away from moisture to prevent clumping.
