Description
Specifications Table
| Product Name | Popso Sesquisodium Salt Buffer Extrapure |
|---|---|
| Pack Size | 25 Gms |
| Form | Powder |
| Grade | Extrapure (98%) |
| Application | Biochemical buffer, pH stabilization, enzyme assays |
Product Description
When precision matters in your lab experiments, the Popso Sesquisodium Salt Buffer Extrapure from SRL Chem delivers unmatched performance. This high-purity (98%) biochemical buffer is designed for researchers, students, and educators who demand consistency in pH-sensitive applications. Whether you’re working on enzyme kinetics, protein studies, or diagnostic assays, this POPSO buffer solution ensures reliable results every time.
POPSO (3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid) is a zwitterionic buffer widely used in biochemistry and molecular biology. Its sesquisodium salt form offers excellent solubility and stability across a broad pH range (7.2–8.5), making it a go-to choice for critical experiments. Unlike traditional buffers, POPSO resists interference from metal ions, ensuring cleaner data in your research.
At eqipped, we understand the challenges of sourcing lab-grade reagents in India. That’s why we’ve partnered with trusted brands like SRL Chem to bring you authentic, high-quality buffers without the hassle of long procurement cycles. Whether you’re a PhD student fine-tuning your thesis or a professor setting up a new lab, this buffer is a smart addition to your inventory.
Upgrade your lab’s buffer system today. Shop Popso Sesquisodium Salt Buffer Extrapure online and experience seamless research workflows.
Why POPSO Buffer Stands Out
Not all buffers are created equal. POPSO’s unique chemical structure offers several advantages over common alternatives like Tris or phosphate buffers:
1. Broad pH Range: Maintains stability between pH 7.2–8.5, ideal for physiological studies and cell culture work.
2. Low Metal Ion Binding: Minimizes interference in enzymatic reactions, ensuring accurate kinetic measurements.
3. High Solubility: Dissolves easily in water, saving you time during preparation.
4. Temperature Stability: Performs consistently across varying lab conditions, reducing experimental variability.
For researchers working with delicate biomolecules, these properties translate to fewer repeated experiments and more trustworthy data. The extrapure grade (98%) further guarantees minimal impurities, so you can focus on your science—not troubleshooting buffer-related issues.
Applications in Your Lab
This versatile buffer finds use in a variety of laboratory settings:
Enzyme Assays: Maintain optimal pH for enzyme activity studies without inhibiting reactions.
Protein Purification: Use as a component in chromatography buffers for stable protein isolation.
Cell Culture Media: Supplement growth media to regulate pH in mammalian cell lines.
Diagnostic Kits: Incorporate into reagent formulations for consistent test performance.
Electrophoresis: Serve as a running buffer for DNA/RNA gel analysis.
Students preparing for practical exams will appreciate its reliability in standard protocols, while seasoned researchers can leverage its precision for publication-quality results.
How to Prepare POPSO Buffer Solution
Getting started is straightforward. Follow this simple protocol to prepare a 0.1 M POPSO buffer (pH 7.4):
1. Weigh out the required amount of Popso Sesquisodium Salt Buffer (check molecular weight for calculations).
2. Dissolve in distilled water to achieve your desired concentration.
3. Adjust pH using NaOH or HCl as needed.
4. Sterilize by filtration (0.22 µm) if required for cell culture applications.
Pro tip: Always store your buffer solution at 4°C and check pH before use, as temperature fluctuations can cause minor shifts.
Safety and Handling
While POPSO is generally non-toxic, proper lab practices ensure safety:
Risk Note: Avoid inhalation or direct contact with eyes/skin. Wear appropriate PPE (gloves, goggles) when handling. In case of contact, rinse thoroughly with water.
Store in a cool, dry place away from incompatible substances. The 25 g pack size is perfect for small-scale labs, reducing waste while ensuring you always have fresh buffer on hand.
Why Researchers Trust eqipped
When you buy lab supplies online, you need a partner who understands your workflow. eqipped simplifies procurement with:
Genuine Products: Directly sourced from SRL Chem, so you receive exactly what’s on the label.
Fast Delivery: Get your buffer in days, not weeks, with our India-wide logistics network.
Transparent Pricing: No hidden fees—just competitive rates for premium reagents.
Expert Support: Have questions about buffer selection? Our team includes lab veterans ready to assist.
We’ve helped thousands of students and researchers across India streamline their supply chain. Join them in making smarter purchasing decisions.
FAQs
What is the ideal pH range for POPSO buffer?
POPSO buffer is most effective between pH 7.2–8.5, making it perfect for physiological studies and enzyme assays requiring near-neutral conditions.
Can I use this buffer for cell culture applications?
Yes, the extrapure grade (98%) is suitable for cell culture when properly sterilized. Always filter through a 0.22 µm membrane before use.
How does POPSO compare to HEPES buffer?
POPSO offers a similar pH range but with lower metal ion binding, making it preferable for experiments involving metal-sensitive enzymes.
What’s the shelf life of Popso Sesquisodium Salt Buffer?
When stored unopened at room temperature, it remains stable for 2 years. After opening, use within 6 months for best results.
Do you offer bulk discounts for educational institutions?
Absolutely! Contact eqipped’s sales team to discuss volume pricing for universities and research labs.
