Description
Specifications Table
| Product Name | Proteinase K Cell Culture Tested |
|---|---|
| Pack Size | 10 mg, 100 mg |
| Form | Lyophilized Powder |
| Grade | Cell Culture Tested |
| Application | DNA/RNA extraction, protein digestion, molecular biology |
Product Description
Proteinase K is a broad-spectrum serine protease widely used in molecular biology for its ability to digest proteins and inactivate nucleases. This cell culture-tested variant ensures minimal contamination, making it ideal for sensitive applications like DNA/RNA extraction, plasmid purification, and tissue dissociation.
Whether you’re a researcher, student, or educator, this enzyme offers consistent performance in labs. Its high purity and stability make it a reliable choice for experiments requiring precise protein degradation. Need Proteinase K for your next experiment? Buy online from eqipped for guaranteed quality and fast shipping.
Proteinase K is commonly used in protocols involving nucleic acid isolation, where it helps eliminate protein contaminants that may interfere with downstream applications. Its robustness under denaturing conditions (e.g., SDS, urea) further enhances its utility in challenging sample preparations.
For researchers working with cell cultures, this variant is tested to ensure compatibility, reducing the risk of cytotoxicity or unwanted side effects. It’s a staple in labs performing PCR, Southern blotting, or other techniques requiring clean nucleic acid samples.
FAQs
What is the optimal temperature for Proteinase K activity?
Proteinase K exhibits peak activity at 50–60°C but remains active across a broad range (20–70°C). For most applications, 37–55°C is ideal.
Can Proteinase K be used for RNA extraction?
Yes, it’s commonly used in RNA isolation protocols to degrade proteins and release nucleic acids. Ensure proper inactivation (e.g., heat or phenol-chloroform) to protect RNA integrity.
How do I inactivate Proteinase K after digestion?
Heat inactivation (95°C for 10 minutes) or using protease inhibitors (e.g., PMSF) are standard methods. For RNA work, phenol-chloroform extraction is preferred.
Is this Proteinase K suitable for cell culture applications?
Yes, this variant is specifically tested for cell culture use, ensuring minimal toxicity and compatibility with mammalian cells.
What’s the shelf life of Proteinase K?
When stored at -20°C, it remains stable for up to 2 years. Check the certificate of analysis for batch-specific expiry details.
