Description
Specifications Table
| Product Name | Proteinase K ex. Tritirachium Album (Type A) for molecular biology, 30U/mg protein | 49936 | SRL Chem |
|---|---|
| Pack Size | 25 Mg, 100 Mg, 500 Mg, 1 Gms, 5 Gms |
| Form | Lyophilized powder |
| Grade | Molecular biology grade |
| Application | DNA/RNA extraction, protein digestion, PCR cleanup |
Product Description
Proteinase K is a broad-spectrum serine protease derived from the fungus Tritirachium album, widely used in molecular biology laboratories for its exceptional ability to digest proteins and inactivate nucleases. With an activity of 30U/mg, this high-purity enzyme is essential for applications like DNA/RNA extraction, PCR cleanup, and protein degradation studies.
Whether you’re a researcher isolating nucleic acids or a student preparing samples for downstream analysis, this Proteinase K ensures reliable performance with minimal contamination. Its stability across a wide pH range (4–12) and resistance to denaturants like SDS make it a versatile tool in your lab arsenal.
At eqipped, we source this enzyme from trusted suppliers like SRL Chem to guarantee consistency and purity. Available in multiple pack sizes (25 Mg to 5 Gms), it caters to both small-scale experiments and bulk requirements.
Upgrade your molecular biology workflow with premium Proteinase K. Order online today for seamless delivery.
FAQs
What is the optimal working concentration for Proteinase K?
Typically, 50–100 µg/mL is used for DNA/RNA extraction, but adjust based on sample type and protocol requirements.
Can this Proteinase K be used for PCR cleanup?
Yes, it effectively degrades proteins that may interfere with PCR amplification, ensuring cleaner results.
Is this enzyme suitable for RNA extraction?
Absolutely. Its broad specificity helps eliminate RNase contamination, protecting RNA integrity.
How do I reconstitute the lyophilized powder?
Dissolve in sterile water or buffer (e.g., 10 mM Tris-HCl, pH 7.5) to the desired concentration. Vortex gently to avoid denaturation.
What safety precautions should I take?
Wear gloves and lab coats. Avoid inhalation or contact with skin/eyes. Dispose of waste according to lab protocols.
