Description
Specifications Table
| Product Name | Proteinase K Solution (20 mg/ml) | 36331 | SRL Chem |
|---|---|
| Pack Size | 1 ml, 5 x1 ml, 5 x5 ml |
| Form | Liquid |
| Grade | Molecular Biology Grade |
| Application | DNA/RNA extraction, protein digestion, molecular biology assays |
Product Description
Proteinase K Solution (20 mg/ml) is a highly active, broad-spectrum serine protease widely used in molecular biology for digesting proteins and inactivating nucleases during nucleic acid extraction. This lab-grade enzyme from SRL Chem ensures consistent performance in critical applications like DNA/RNA purification, PCR cleanup, and tissue lysis.
Whether you’re a student setting up your first experiment or a researcher optimizing protocols, this solution delivers reliable results. Its stability across a wide pH and temperature range makes it versatile for various lab workflows. Need high-quality Proteinase K for your next experiment? Buy online at eqipped for fast delivery and competitive pricing.
Key Applications
Proteinase K Solution is essential for:
- Isolating intact DNA/RNA from tissues or cells
- Removing protein contaminants in nucleic acid preparations
- Inactivating nucleases to protect target molecules
- Preparing samples for PCR, sequencing, or blotting
Why This Solution Stands Out
This formulation offers several advantages over generic alternatives:
- Pre-diluted to 20 mg/ml for convenience
- High purity minimizes background interference
- Stable in common lab buffers
- Suitable for both manual and automated protocols
Storage and Handling
Store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles. For working solutions, aliquot and store at 4°C for up to 1 month. Always wear appropriate PPE when handling.
Safety Note
Proteinase K is a potent enzyme. Handle with care to avoid skin/eye contact. In case of exposure, rinse immediately with water. Dispose of waste according to local regulations.
FAQs
What is Proteinase K used for in DNA extraction?
It digests proteins and inactivates nucleases, ensuring clean nucleic acid isolation without degradation.
How long does Proteinase K remain active?
At 37°C, it retains activity for hours. For overnight digestions, use 50-60°C to enhance efficiency.
Can I use this for RNA extraction?
Yes, but ensure RNase-free conditions. Proteinase K effectively removes RNA-binding proteins.
What’s the shelf life of this solution?
Unopened vials last 2 years at -20°C. After opening, use within 1 month when stored at 4°C.
Where to buy Proteinase K online in India?
Order authentic SRL Chem Proteinase K Solution (20 mg/ml) at eqipped for guaranteed quality and fast shipping.
