Description
Specifications Table
| Product Name | r-Trypsin – Type Standard ACF ex. Porcine for cell culture |
|---|---|
| Quantity/Pack Size | 100 Mg, 1 Gms, 5 Gms |
| Form | Lyophilized powder |
| Grade | Cell culture grade |
| Application | Cell dissociation, tissue culture, research |
Product Description
When it comes to cell culture, the quality of your reagents can make or break your experiments. That’s where r-Trypsin – Type Standard ACF ex. Porcine steps in. This high-purity trypsin, sourced from porcine pancreas, is specifically designed for cell dissociation and tissue culture applications. With an activity of 800U/mg and an ultra-low endotoxin level of 0.05EU/mg, it ensures minimal contamination and reliable performance in sensitive cell lines.
Whether you’re a student setting up your first cell culture experiment or a researcher working on critical projects, this trypsin offers consistency and efficiency. Its endotoxin-free nature makes it suitable for applications where bacterial contamination is a concern, such as primary cell isolation or stem cell research. The lyophilized powder form ensures stability and easy reconstitution, giving you flexibility in your lab workflow.
At eqipped, we understand the importance of precision in research. That’s why we provide r-Trypsin in multiple pack sizes—100mg, 1g, and 5g—so you can choose the right quantity for your needs without unnecessary waste. Whether you’re running small-scale experiments or large batch cultures, this trypsin delivers the performance you expect.
Upgrade your cell culture workflow with high-purity trypsin. Buy r-Trypsin online today for consistent, contamination-free results.
FAQs
What is the difference between porcine trypsin and recombinant trypsin?
Porcine trypsin is derived from pig pancreas and is widely used due to its cost-effectiveness and high activity. Recombinant trypsin, on the other hand, is produced in labs using genetic engineering, which can offer higher purity but at a higher cost. For most cell culture applications, porcine trypsin like this one (800U/mg) is sufficient and reliable.
Can this trypsin be used for primary cell isolation?
Yes, this endotoxin-free trypsin (0.05EU/mg) is suitable for primary cell isolation. Its low endotoxin levels minimize the risk of activating immune responses in sensitive primary cells, making it a safe choice for such applications.
How do I reconstitute this lyophilized trypsin?
Reconstitute the powder in sterile PBS or HBSS to the desired concentration (typically 0.05% to 0.25%). Gently swirl to dissolve—avoid vigorous shaking to prevent denaturation. Once reconstituted, filter-sterilize if needed and store aliquots at -20°C.
What is the shelf life of this trypsin?
The lyophilized powder has a shelf life of 2 years when stored at 2–8°C. Once reconstituted, it should be used within 1 month if stored at 4°C or 6 months if stored at -20°C. Always check for any signs of contamination before use.
Is this trypsin suitable for GMP or clinical applications?
This trypsin is research-grade and not intended for clinical or GMP applications. For such uses, you would need a clinical-grade or GMP-certified trypsin, which undergoes additional testing and validation.
