Description
Specifications Table
| Product Name | Rhodamine B-Iso-Thiocyanate | 214165 | CDH |
|---|---|
| Quantity/Pack Size | 100 mg or 1 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Fluorescence microscopy, biochemical labeling |
Product Description
Rhodamine B-Iso-Thiocyanate is a versatile fluorescent dye widely used in research and diagnostic applications. Its bright red fluorescence makes it ideal for labeling proteins, antibodies, and other biomolecules in microscopy and flow cytometry. Whether you’re a student setting up a fluorescence experiment or a researcher needing consistent results, this dye offers excellent photostability and high quantum yield.
At eqipped, we ensure that every batch of Rhodamine B-Iso-Thiocyanate meets strict quality standards, so you can trust its performance in your lab. The powder form allows for easy dissolution in organic solvents or aqueous buffers, depending on your experimental needs. Many researchers prefer this dye for its strong emission in the orange-red spectrum, which minimizes overlap with other common fluorophores.
If you’re looking to buy Rhodamine B-Iso-Thiocyanate online, eqipped provides a seamless purchasing experience with fast shipping across India. Our packaging ensures stability during transit, so you receive the product in optimal condition. Whether you need it for cell imaging, immunoassays, or other fluorescence-based techniques, this dye delivers reliable results every time.
Applications of Rhodamine B-Iso-Thiocyanate
This fluorescent dye is commonly used in:
- Fluorescence microscopy for cell and tissue staining
- Protein and antibody labeling in biochemical assays
- Flow cytometry for cell analysis
- Immunofluorescence studies
- DNA and RNA labeling in molecular biology
Its high extinction coefficient and strong fluorescence make it a preferred choice for researchers who need bright, stable signals in their experiments. The isothiocyanate group allows for easy conjugation with amino groups, making it a go-to reagent for labeling biomolecules.
Why Researchers Trust Rhodamine B-Iso-Thiocyanate
One of the key reasons this dye is so popular in labs is its versatility. Unlike some fluorophores that fade quickly under excitation, Rhodamine B-Iso-Thiocyanate maintains its intensity, allowing for longer imaging sessions. This is especially useful in time-lapse microscopy or high-throughput screening.
Another advantage is its compatibility with standard filter sets used in fluorescence microscopes. Most labs already have the necessary equipment to detect its emission, so no additional investments are needed. This makes it a cost-effective choice for both academic and industrial research.
Students and educators also appreciate its ease of use. The straightforward conjugation process means even those new to fluorescence techniques can achieve good results. Many university labs stock this dye for teaching purposes, as it provides clear, visible outcomes in experiments.
Handling and Storage
To ensure the longevity of your Rhodamine B-Iso-Thiocyanate, store it in a cool, dry place away from direct light. The powder is stable at room temperature, but for long-term storage, keeping it at 2-8°C is recommended. Always handle the dye with care, as it can stain surfaces and skin.
When preparing solutions, use appropriate solvents like DMSO or ethanol, depending on your protocol. Avoid prolonged exposure to light during experiments to prevent photobleaching. Proper handling ensures you get the best performance from this high-quality dye.
Safety Considerations
Rhodamine B-Iso-Thiocyanate is a chemical dye and should be handled with standard lab safety precautions. Wear gloves, goggles, and a lab coat when working with it. Avoid inhalation or ingestion, and dispose of waste according to local regulations. Always refer to the Safety Data Sheet (SDS) for detailed handling instructions.
FAQs
What is Rhodamine B-Iso-Thiocyanate used for?
It is primarily used as a fluorescent label in microscopy, flow cytometry, and biochemical assays due to its bright red fluorescence.
How do I dissolve Rhodamine B-Iso-Thiocyanate?
It can be dissolved in organic solvents like DMSO or ethanol, or in aqueous buffers depending on the application.
Is this dye compatible with live-cell imaging?
Yes, but ensure proper concentration and exposure times to minimize phototoxicity.
Can I buy Rhodamine B-Iso-Thiocyanate in bulk?
Yes, eqipped offers both 100 mg and 1 gm pack sizes. Contact us for larger quantities.
What is the shelf life of Rhodamine B-Iso-Thiocyanate?
When stored properly, it remains stable for at least 2 years from the date of manufacture.
