Description
Specifications Table
Product Name – Safranin O (biological stain) Indicator
Quantity/Pack Size – 25 g
Form – Powder
Grade – Laboratory
Application – Gram staining, histology, microscopy
Product Overview
Safranin O is a cationic phenazine dye widely used as a biological stain in microbiology and histology laboratories. This high-purity powder exhibits excellent solubility in water and ethanol, making it ideal for preparing staining solutions. The dye binds selectively to gram-positive bacteria, lignin in plant tissues, and nucleic acids, producing a distinctive red or pink coloration under microscopic examination. Its stable chemical structure ensures consistent staining results across various pH ranges (typically 6.0-8.0). The laboratory-grade Safranin O offered here meets stringent quality standards for research applications, with minimal impurities that could interfere with staining protocols. The powder form allows for flexible preparation of working solutions at different concentrations (commonly 0.1-1% w/v). When used as a counterstain in gram staining procedures, it provides clear differentiation between gram-positive and gram-negative bacteria. The dye’s fluorescence properties under UV light further enhance its utility in advanced microscopy techniques. Proper storage in airtight containers protects against moisture absorption and maintains staining efficacy over extended periods.
FAQs
1. What is the ideal concentration for preparing Safranin O staining solution?
A 0.5-1% w/v solution in distilled water or 95% ethanol works well for most applications, though concentrations may vary based on specific protocols.
2. Can Safranin O be used for both bacterial and plant tissue staining?
Yes, it effectively stains gram-positive bacteria red/pink and lignified plant cell walls, though preparation methods differ slightly between applications.
3. How should Safranin O powder be stored for long-term use?
Keep in a tightly sealed container at room temperature, protected from light and moisture to prevent degradation of staining properties.
4. What alternatives exist for gram-positive bacterial staining?
Crystal violet or methylene blue can serve as alternatives, though they may require protocol adjustments for comparable results.
5. Does Safranin O require any special disposal procedures?
While not classified as hazardous, standard laboratory waste disposal protocols should be followed, avoiding release into water systems.
