Description
Specifications Table
Product Name – Sodium phosphate, tribasic dodecahydrate, 98%, for biochemistry
Quantity/Pack Size – 2.5KG, 500GR
Form – Crystalline powder
Grade – 98% purity, biochemistry grade
Application – pH buffering, molecular biology, protein purification
Product Overview
Sodium phosphate tribasic dodecahydrate (98% purity) is a highly refined, water-soluble crystalline powder designed for precision biochemistry applications. Its stable pH buffering capacity makes it indispensable in molecular biology protocols, including DNA/RNA extraction, protein purification, and enzyme assays. The dodecahydrate form ensures optimal hydration and dissolution, eliminating clumping or residue formation. This grade is rigorously tested for low heavy metal and endotoxin contamination, guaranteeing reproducibility in sensitive experiments. The compound’s high solubility in water (1 in 4 parts) allows for rapid preparation of phosphate buffers across a wide pH range (pH 11–12 in 1% solution). Its non-hygroscopic nature ensures long-term stability when stored under recommended conditions. Compatible with most lab-grade reagents, it serves as a reliable alternative to sodium hydroxide for alkaline buffering without introducing caustic risks. Ideal for researchers requiring consistent ionic strength and minimal batch-to-batch variability.
FAQs
1. What is the solubility of sodium phosphate tribasic dodecahydrate in water?
It dissolves readily in water at a ratio of 1:4 (1 part solute to 4 parts solvent), forming a clear, colorless solution with a pH of 11–12 at 1% concentration.
2. Can this be used as a substitute for disodium phosphate in buffer preparation?
Yes, but adjust the molar ratios accordingly, as tribasic phosphate has a higher pH and different ionization profile compared to disodium phosphate.
3. How should this product be stored to maintain stability?
Store in a tightly sealed container at room temperature (15–30°C), protected from moisture and direct sunlight to prevent hydration loss or caking.
4. Is this grade suitable for cell culture applications?
While primarily designed for biochemistry, it can be used in cell culture if further sterilized (e.g., filtration) and tested for endotoxin levels below your protocol’s threshold.
5. What impurities are typically present in this 98% grade?
Trace amounts of sodium carbonate, heavy metals (e.g., lead, arsenic <10 ppm), and insoluble matter may be present but are minimized to meet biochemistry-grade standards.
