Description
Specifications Table
| Product Name | TAPS Buffer 99.0% |
|---|---|
| Pack Size | 25g / 100g / 500g |
| Form | Powder |
| Grade | Analytical |
| Application | Biochemical research, protein studies, electrophoresis |
Product Description
TAPS buffer, or 3-[[tris(hydroxymethyl)methyl]amino]propanesulfonic acid, is a high-purity biochemical reagent widely used in laboratories for maintaining stable pH conditions. With a purity of 99.0%, this buffer is ideal for applications requiring precise pH control, such as protein purification, enzyme assays, and gel electrophoresis. Its zwitterionic nature makes it highly effective in biological systems, ensuring minimal interference with experimental results.
Whether you’re a student setting up your first experiment or a researcher fine-tuning a protocol, TAPS buffer from eqipped provides the reliability you need. The analytical grade ensures consistency, while the powder form allows for flexible preparation of solutions at your desired concentration.
For labs in India, sourcing high-quality buffers like TAPS can be challenging. eqipped simplifies this by offering authentic CDH products with fast shipping and competitive pricing. Whether you need a small quantity for a single experiment or bulk supplies for ongoing research, you can buy TAPS buffer online with confidence.
Why TAPS Buffer is Essential for Your Lab
In biochemical research, maintaining the correct pH is critical. TAPS buffer stands out because of its excellent buffering capacity in the pH range of 7.7 to 9.1, making it a go-to choice for experiments involving proteins and nucleic acids. Unlike some buffers that can interfere with biological processes, TAPS is biologically inert, ensuring your results remain accurate.
Students working on molecular biology projects will find TAPS buffer particularly useful for DNA and RNA studies. Educators can rely on it for consistent results in classroom demonstrations. Meanwhile, researchers in enzymology and structural biology depend on its stability for reproducible data.
How to Prepare TAPS Buffer Solutions
Preparing a TAPS buffer solution is straightforward. Start by dissolving the required amount of powder in deionized water. For a 1M solution, add 243.3 grams of TAPS buffer to 1 liter of water. Adjust the pH using HCl or NaOH as needed. Always use a calibrated pH meter for accuracy.
For most applications, a 50mM to 100mM concentration works well. Store the prepared solution at room temperature for short-term use or at 4°C for longer storage. Avoid repeated freeze-thaw cycles to maintain buffer integrity.
Applications of TAPS Buffer in Research
TAPS buffer is versatile and finds use in various laboratory applications. In protein research, it helps maintain the native structure of proteins during purification and crystallization. It’s also commonly used in electrophoresis, where stable pH is crucial for separating molecules based on size and charge.
In molecular biology, TAPS buffer is often employed in DNA hybridization and blotting techniques. Its compatibility with common lab reagents makes it a preferred choice for many protocols. Additionally, it’s used in cell culture media where precise pH control is necessary for cell viability.
Safety and Handling
While TAPS buffer is generally safe to handle, it’s important to follow standard laboratory practices. Wear appropriate personal protective equipment, including gloves and safety goggles, when preparing solutions. Avoid inhaling the powder, and work in a well-ventilated area.
In case of skin or eye contact, rinse thoroughly with water. For accidental ingestion, seek medical attention immediately. Always refer to the Safety Data Sheet (SDS) for detailed handling and disposal instructions.
Choosing the Right Pack Size
eqipped offers TAPS buffer in multiple pack sizes to suit different needs. The 25g pack is perfect for students and small-scale experiments, while the 100g and 500g options are ideal for research labs with higher consumption. Buying in bulk not only ensures you have enough buffer for multiple experiments but also reduces the cost per gram.
If you’re unsure about the quantity you need, consider the frequency of your experiments and the volume of buffer required per test. For most labs, the 100g pack strikes a good balance between cost and convenience.
Where to Buy TAPS Buffer Online in India
Finding a reliable supplier for lab chemicals can be time-consuming. eqipped makes it easy to buy TAPS buffer online with just a few clicks. As a trusted supplier of CDH products, we ensure authenticity and quality in every order. Our user-friendly platform allows you to browse, select, and order with confidence.
With fast shipping across India, you can get your TAPS buffer delivered to your lab or institution without delays. We also offer secure payment options and responsive customer support to assist you with any queries.
FAQs
What is TAPS buffer used for?
TAPS buffer is primarily used in biochemical research for maintaining stable pH conditions. It’s commonly employed in protein studies, enzyme assays, and electrophoresis due to its effective buffering range of 7.7 to 9.1.
How do I prepare a 50mM TAPS buffer solution?
To prepare a 50mM solution, dissolve 12.165 grams of TAPS buffer in 1 liter of deionized water. Adjust the pH using HCl or NaOH as needed, and verify with a calibrated pH meter.
Is TAPS buffer compatible with cell culture?
Yes, TAPS buffer is biologically inert and can be used in cell culture media where precise pH control is necessary. However, always test compatibility with your specific cell lines.
What is the shelf life of TAPS buffer?
When stored properly in a cool, dry place, TAPS buffer powder has a long shelf life. Prepared solutions should be stored at 4°C and used within a few months for best results.
Can I buy TAPS buffer in bulk from eqipped?
Yes, eqipped offers TAPS buffer in bulk pack sizes of 100g and 500g, making it convenient for labs with high consumption. Bulk purchases also provide better value for money.
