Description
Specifications Table
| Product Name | TAPSO, for biochemistry | 327810250 | Thermofisher |
|---|---|
| Pack Size | 25GR, 100GR, 500GR |
| Form | Powder |
| Grade | Biochemistry Grade |
| Application | pH buffering in biochemical assays, protein research |
Product Description
TAPSO (3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid) is a zwitterionic buffer widely used in biochemistry and molecular biology. Known for its excellent pH stability and minimal interference with biological reactions, TAPSO is a preferred choice for researchers working with enzymes, proteins, and cell cultures. Whether you’re conducting enzymatic assays or studying protein interactions, this high-purity buffer ensures consistent and reliable results.
At eqipped, we understand the importance of precision in your experiments. That’s why we offer TAPSO buffer in multiple pack sizes to suit your lab’s needs. From small-scale research to bulk requirements, you can buy TAPSO buffer online with confidence, knowing you’re getting a product trusted by scientists worldwide.
Need a dependable buffer for your next experiment? Buy TAPSO buffer online today and experience seamless performance in your research.
TAPSO buffer is particularly useful in applications where maintaining a stable pH between 7.0 and 8.2 is critical. Its low toxicity and compatibility with most biological systems make it an ideal choice for delicate experiments. Researchers often use TAPSO in place of other buffers like HEPES or MOPS when they need a buffer with a different pKa or when working with systems sensitive to certain buffer components.
When you buy TAPSO buffer from eqipped, you’re not just purchasing a chemical you’re investing in the quality and reproducibility of your research. Our TAPSO buffer comes in sealed packaging to prevent contamination and ensure long-term stability. Whether you’re a student setting up your first experiment or a seasoned researcher optimizing a protocol, this buffer will help you achieve accurate and reproducible results.
Applications of TAPSO Buffer
TAPSO buffer finds extensive use in various biochemical applications. In protein research, it helps maintain the native conformation of proteins during purification and characterization. For enzymatic assays, TAPSO provides a stable environment that allows enzymes to function at their optimal pH. Cell culture studies also benefit from TAPSO’s ability to maintain physiological pH conditions, supporting cell viability and growth.
The buffer’s versatility extends to DNA and RNA studies, where maintaining the correct pH is crucial for the stability of nucleic acids. TAPSO is also commonly used in electrophoresis applications, helping to create the right conditions for the separation of biomolecules. Its compatibility with various detection methods makes it a valuable tool in analytical biochemistry.
Why TAPSO Buffer Stands Out
What sets TAPSO buffer apart from other buffering agents is its unique combination of properties. Unlike some buffers that can interfere with metal ions or other reaction components, TAPSO maintains its buffering capacity without disrupting biological processes. This makes it particularly valuable in experiments where metal ion interactions are important.
The buffer’s temperature stability is another advantage. TAPSO maintains its pKa across a range of temperatures, making it suitable for experiments that require temperature variations. This property is especially useful in enzymatic studies where temperature can significantly affect reaction rates.
Choosing the Right Pack Size
Selecting the appropriate pack size is crucial for both cost-effectiveness and experimental needs. The 25GR pack is ideal for small-scale experiments or when you need to test different buffer conditions. For regular laboratory use, the 100GR pack offers a good balance between quantity and storage convenience. Research facilities with high throughput or large-scale experiments will find the 500GR pack most economical.
Remember that proper storage is key to maintaining the buffer’s quality. Store TAPSO buffer in a cool, dry place, away from direct sunlight. Once prepared, buffer solutions should be stored according to your laboratory’s standard protocols to prevent contamination.
Safety and Handling
While TAPSO buffer is generally considered safe for laboratory use, it’s important to follow standard laboratory safety practices. Always wear appropriate personal protective equipment, including gloves and safety glasses, when handling the powder form. Work in a well-ventilated area or under a fume hood when preparing buffer solutions.
In case of accidental ingestion or contact with eyes, rinse immediately with plenty of water and seek medical advice. Keep the buffer out of reach of children and store it separately from food and drink. Proper disposal of unused buffer and its containers should follow your institution’s chemical waste disposal guidelines.
FAQs
What is the pKa of TAPSO buffer?
TAPSO buffer has a pKa of approximately 7.6 at 25°C, making it ideal for maintaining physiological pH conditions in biochemical experiments.
Can TAPSO buffer be used in cell culture?
Yes, TAPSO buffer is commonly used in cell culture applications due to its low toxicity and ability to maintain stable pH conditions that support cell viability.
How do I prepare a 1M solution of TAPSO buffer?
To prepare a 1M solution, dissolve 22.33 grams of TAPSO powder in water, adjust the pH as needed with HCl or NaOH, and bring the final volume to 100 mL with distilled water.
Is TAPSO buffer compatible with metal ions?
TAPSO buffer generally doesn’t chelate metal ions, making it suitable for experiments where metal ion interactions are
