Description
Specifications Table
| Product Name | Tetrathionate Brilliant Green Bile Broth, Granulated |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Granulated |
| Grade | For Microbiology |
| Application | Selective enrichment of Salmonella spp. |
Product Description
Tetrathionate Brilliant Green Bile Broth (GM1255) is a specialized microbiological medium designed for the selective enrichment of Salmonella species from food, water, and environmental samples. This granulated broth combines tetrathionate and brilliant green to inhibit the growth of competing bacteria while promoting Salmonella proliferation.
The medium works by suppressing gram-positive organisms and coliforms, allowing Salmonella to thrive. Its formulation adheres to standard microbiological protocols, making it ideal for research labs, educational institutions, and quality control testing.
For researchers and students working on food safety or clinical microbiology projects, this broth provides reliable results when used as part of standard Salmonella detection workflows. The 500 gm pack size offers sufficient quantity for multiple tests, ensuring cost-effectiveness for regular use.
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How to Use Tetrathionate Brilliant Green Bile Broth
1. Reconstitute the granulated medium as per manufacturer instructions (typically 40.5 gm per liter of distilled water).
2. Sterilize by autoclaving at 121°C for 15 minutes.
3. Inoculate the sample and incubate at 35-37°C for 18-24 hours.
4. Subculture onto selective agar for confirmation.
Storage and Handling
Store the granulated powder in a cool, dry place away from direct sunlight. Reconstituted broth should be used immediately or stored at 2-8°C for short-term use. Always follow standard microbiological safety practices when handling.
Why This Medium is Essential for Salmonella Testing
Salmonella detection requires selective enrichment to isolate the pathogen from complex samples. This broth’s unique combination of inhibitors ensures that only Salmonella can grow, reducing false negatives. It’s particularly useful in:
- Food safety testing
- Water quality analysis
- Clinical microbiology research
- Educational lab experiments
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Safety Information
This product is for professional laboratory use only. Handle with standard microbiological precautions. Avoid inhalation or direct contact with skin and eyes. Dispose of used media according to biohazard waste protocols.
FAQs
What is the shelf life of Tetrathionate Brilliant Green Bile Broth?
The granulated powder typically has a shelf life of 2-3 years when stored properly. Always check the expiration date on the packaging.
Can this broth detect all Salmonella serotypes?
While highly effective for most Salmonella species, some serotypes may require additional confirmation steps. Always follow standard microbiological protocols.
What’s the difference between this and other Salmonella enrichment broths?
This medium specifically uses tetrathionate and brilliant green, which provides stronger inhibition of competing flora compared to general enrichment broths.
Is this product suitable for student lab experiments?
Yes, it’s commonly used in educational settings for demonstrating Salmonella enrichment techniques. Proper supervision is recommended.
How quickly can I get this product delivered?
eqipped offers fast shipping across India. Delivery times vary by location, typically 3-7 business days.
