Description
Specifications Table
| Product Name | TMB Substrate for ELISA | TCL278-100ML | Himedia |
|---|---|
| Quantity/Pack Size | 100 ml |
| Form | Liquid |
| Grade | Research Grade |
| Application | ELISA assays, immunological testing |
Product Description
When precision matters in your ELISA experiments, the TMB Substrate for ELISA by Himedia (TCL278-100ML) delivers consistent, high-sensitivity results. This ready-to-use liquid substrate is formulated to provide a strong blue color reaction upon enzymatic cleavage, making it ideal for quantitative and qualitative immunological assays. Whether you’re a student conducting academic research or a professional in a diagnostic lab, this substrate ensures reliable detection with minimal background noise.
Designed for convenience, this TMB substrate eliminates the need for preparation, saving you valuable time in the lab. Its stable formulation guarantees reproducibility across multiple experiments, which is critical for research integrity. The 100 ml pack size is perfect for frequent users, offering cost-effective value without compromising on quality.
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For researchers and educators, this substrate is particularly useful in teaching environments where consistency and ease of use are paramount. The clear color development allows students to easily interpret results, making it a preferred choice in academic settings. Additionally, its compatibility with standard ELISA protocols ensures seamless integration into existing laboratory workflows.
At eqipped, we understand the importance of reliable reagents in scientific research. That’s why we source only the best products, like this Himedia TMB substrate, to support your work. Whether you’re setting up a new lab or restocking essential supplies, you can trust eqipped for authentic, high-performance lab consumables.
FAQs
What is TMB substrate used for in ELISA?
TMB (3,3′,5,5′-Tetramethylbenzidine) substrate is widely used in ELISA assays as a chromogenic substrate for horseradish peroxidase (HRP). It produces a blue color during the enzymatic reaction, which changes to yellow after adding a stop solution, allowing accurate measurement of the target analyte.
How should TMB substrate be stored?
Store the TMB substrate at 2–8°C in its original container, protected from direct light. Do not freeze the product, and always follow the manufacturer’s storage recommendations to maintain optimal performance.
Is this TMB substrate compatible with all HRP-based ELISA kits?
This TMB substrate is compatible with most ELISA protocols that use horseradish peroxidase (HRP)-conjugated antibodies or enzymes. However, users should confirm compatibility with their specific assay protocol before use.
Can this TMB substrate be used for applications other than ELISA?
Yes, in addition to ELISA, TMB substrate can be used in other HRP-based detection methods, including immunohistochemistry, Western blotting, and enzyme immunoassays, provided the experimental protocol supports its use.
What is the shelf life of TMB substrate?
When stored under the recommended conditions, TMB substrate typically remains stable until the manufacturer’s stated expiry date. Always check the product label and avoid using expired reagents for reliable assay results.
