Description
Specifications Table
Product Name – Trifluoroacetic acid (for peptide synthesis)
Quantity/Pack Size – 1 L
Form – Liquid
Grade – Peptide Synthesis Grade
Application – Peptide synthesis, HPLC, mass spectrometry
Product Overview
Trifluoroacetic acid (TFA) is a highly purified, peptide synthesis-grade reagent designed for critical applications in biochemical research. With a minimum purity of 99%, this colorless liquid ensures reliable performance in peptide cleavage, deprotection, and purification processes. Its low moisture content (<0.1%) minimizes side reactions, making it ideal for high-performance liquid chromatography (HPLC) and mass spectrometry. The acid's strong ionizing properties facilitate efficient protonation, while its volatility allows for easy removal during sample preparation. Packaged in amber glass bottles to prevent photodegradation, this TFA maintains stability under standard laboratory conditions. The reagent is compatible with most organic solvents and resin systems used in solid-phase peptide synthesis (SPPS). Its consistent quality and batch-to-batch reproducibility make it a preferred choice for researchers requiring precise control over reaction conditions. The product undergoes rigorous quality testing to ensure absence of metal impurities and residual solvents that could interfere with sensitive analytical techniques.
FAQs
1. What is the typical shelf life of this TFA when stored properly?
When stored in its original tightly sealed container at room temperature, away from moisture and direct sunlight, this trifluoroacetic acid maintains its specified purity for at least 24 months from the date of manufacture.
2. Can this TFA be used with Fmoc chemistry in peptide synthesis?
Yes, this peptide synthesis-grade TFA is fully compatible with Fmoc chemistry protocols, providing efficient cleavage of peptide-resin linkages while minimizing side reactions that could compromise peptide integrity.
3. What safety precautions should be taken when handling this acid?
Always handle TFA in a well-ventilated fume hood wearing appropriate PPE including acid-resistant gloves, safety goggles, and lab coat. The reagent is highly corrosive and volatile, requiring careful handling to prevent inhalation or skin contact.
4. How does this TFA compare to reagent-grade TFA for analytical applications?
This peptide synthesis-grade TFA undergoes additional purification steps to remove trace impurities that could interfere with mass spectrometry or HPLC analysis, making it superior to standard reagent-grade TFA for sensitive applications.
5. What is the recommended disposal method for unused TFA?
Neutralize with a compatible base like sodium bicarbonate solution before disposal according to local hazardous waste regulations. Never dispose of untreated TFA in regular waste streams or drainage systems.
