Description
Specifications Table
| Product Name | Triple Sugar Saline Iron Agar |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Microbiology, Bacterial Identification |
Product Description
Triple Sugar Iron Agar (TSI Agar) is a versatile differential medium widely used in microbiology labs for identifying and differentiating enteric bacteria. This medium contains three sugars—glucose, sucrose, and lactose—along with iron salts to detect hydrogen sulfide production. The inclusion of phenol red as a pH indicator helps visualize acid production from carbohydrate fermentation.
TSI Agar is particularly useful for distinguishing between Salmonella, Shigella, and other gram-negative bacteria. The medium’s unique formulation allows for the observation of both acid and gas production, making it an essential tool for clinical and research laboratories.
At eqipped, we provide high-quality TSI Agar from Himedia, ensuring reliable results for your microbiological studies. Whether you’re a student, researcher, or educator, this product is designed to meet your laboratory needs with precision.
How to Use Triple Sugar Iron Agar
To prepare TSI Agar, suspend 500 grams of the powder in 1 liter of distilled water and sterilize by autoclaving at 121°C for 15 minutes. After cooling, dispense the medium into sterile tubes and allow it to solidify in a slant position. Inoculate the medium with a bacterial sample and incubate at 35-37°C for 18-24 hours. Observe the color changes in the butt and slant to interpret the results.
Buy Triple Sugar Iron Agar online today for accurate bacterial identification in your lab experiments.
Applications of TSI Agar
TSI Agar is commonly used in:
- Clinical microbiology for identifying enteric pathogens
- Research laboratories for bacterial fermentation studies
- Educational institutions for teaching microbiology techniques
Storage and Handling
Store the powdered medium in a cool, dry place away from direct sunlight. Once prepared, the agar slants should be stored at 2-8°C and used within a reasonable timeframe to ensure optimal performance.
Risk Note
This product is for laboratory use only. Handle with care and follow standard laboratory safety protocols. Avoid inhalation or direct contact with skin and eyes.
FAQs
What is Triple Sugar Iron Agar used for?
TSI Agar is used to differentiate enteric bacteria based on their ability to ferment sugars and produce hydrogen sulfide.
How do I interpret TSI Agar results?
Yellow slant and butt indicate acid production, while blackening suggests hydrogen sulfide production. Refer to standard microbiology guides for detailed interpretation.
Can I buy TSI Agar in smaller quantities?
Yes, eqipped offers TSI Agar in various pack sizes to suit your laboratory needs.
Is TSI Agar suitable for student labs?
Absolutely! TSI Agar is widely used in educational settings for teaching bacterial identification techniques.
How long does TSI Agar last after preparation?
Prepared TSI Agar slants should be used within a few weeks for best results. Always check for signs of contamination before use.
