Specifications Table

Product Name – Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3
Quantity/Pack Size – 1LT
Form – Liquid
Grade – Molecular Biology
Application – Gel electrophoresis, nucleic acid separation

Product Overview

This 10x Tris-Borate-EDTA (TBE) buffer is a high-purity solution specifically formulated for molecular biology applications, offering superior performance in nucleic acid electrophoresis. The buffer maintains a stable pH of 8.3, ensuring optimal conditions for DNA and RNA separation with minimal ion interference. Its composition includes EDTA, which chelates divalent metal ions to prevent nuclease activity, while the absence of DNase, RNase, and protease contaminants guarantees sample integrity. The concentrated 10x formulation allows for convenient dilution to working strength, reducing storage space requirements while maintaining consistency across experiments. Designed for researchers requiring reproducible results, this TBE buffer provides excellent buffering capacity and conductivity, producing sharp, well-resolved bands in agarose and polyacrylamide gels. The liquid form eliminates the need for dissolution, saving preparation time, while its sterile filtration ensures contamination-free performance. Ideal for high-throughput laboratories, this buffer supports a wide range of molecular biology protocols where nucleic acid purity and resolution are critical.

FAQs

1. What is the shelf life of this TBE buffer when stored properly?

When stored at room temperature in its original sealed container, this 10x TBE buffer maintains stability for 24 months from the date of manufacture.

2. Can this buffer be autoclaved for additional sterilization?

Autoclaving is not recommended as it may cause precipitation of buffer components and alter the pH, potentially affecting performance in electrophoresis.

3. How does this TBE buffer compare to TAE buffer for DNA separation?

TBE buffer offers higher buffering capacity and better resolution for smaller DNA fragments compared to TAE, though it may require longer run times for larger fragments.

4. Is this buffer compatible with SYBR Safe or ethidium bromide staining?

Yes, this nuclease-free TBE buffer is fully compatible with both SYBR Safe and ethidium bromide for nucleic acid visualization under UV light.

5. What dilution ratio should be used for preparing working solution?

Dilute the 10x concentrate with deionized water at a 1:9 ratio to prepare a 1x working solution for standard electrophoresis applications.