Description
Specifications Table
| Product Name | Tris Buffer Superior Extrapure AR, ACS, 99.9% |
|---|---|
| Quantity/Pack Size | 100 Gms, 500 Gms, 5 Kg |
| Form | Powder |
| Grade | AR, ACS, Extrapure |
| Application | Molecular biology, pH buffering, biochemical assays |
Product Description
Tris Buffer, also known as Tris(hydroxymethyl)aminomethane, is a widely used buffering agent in molecular biology and biochemistry. This Superior Extrapure AR, ACS, 99.9% grade ensures high purity and consistency, making it ideal for critical applications such as DNA/RNA extraction, protein purification, and enzyme assays.
Whether you’re a student setting up a lab experiment or a researcher conducting advanced studies, this Tris Buffer provides stable pH control in the range of 7.0–9.0. Its high solubility in water and compatibility with various biological systems make it a staple in laboratories across India.
At eqipped, we source this product from SRL Chem, a trusted name in laboratory chemicals, ensuring you receive a product that meets international quality standards. The AR (Analytical Reagent) and ACS (American Chemical Society) grades guarantee minimal impurities, which is crucial for sensitive experiments.
Looking for a reliable Tris Buffer supplier? Buy online from eqipped for authentic, lab-grade chemicals delivered to your doorstep.
Key Applications
This Tris Buffer is commonly used in:
- DNA and RNA extraction protocols
- Protein electrophoresis (SDS-PAGE, Western blotting)
- Enzyme activity assays
- Cell culture media preparation
- PCR and other molecular biology techniques
Why This Grade Matters
The Extrapure AR, ACS, 99.9% grade is essential for experiments where even minor contaminants can affect results. Unlike lower-grade buffers, this product undergoes rigorous purification to remove traces of metals, nucleases, and other impurities that could interfere with your work.
For researchers and educators, this means fewer failed experiments and more reproducible data. Students can also benefit from using high-quality reagents early in their training, ensuring they develop good lab practices.
Handling and Storage
To maintain the integrity of your Tris Buffer:
- Store in a cool, dry place away from direct sunlight.
- Keep the container tightly sealed to prevent moisture absorption.
- Use clean, dry spatulas to avoid contamination.
Need bulk quantities for your lab? eqipped offers Tris Buffer in 100 Gms, 500 Gms, and 5 Kg packs to suit your needs.
Safety Information
While Tris Buffer is generally safe, it’s important to follow standard lab safety protocols:
- Wear appropriate PPE (gloves, lab coat, goggles).
- Avoid inhalation or ingestion.
- Dispose of waste according to local regulations.
FAQs
What is the shelf life of Tris Buffer?
When stored properly, Tris Buffer has a shelf life of 2–3 years. Always check the expiration date on the packaging.
Can I use this Tris Buffer for PCR?
Yes, this Extrapure AR, ACS grade is suitable for PCR and other molecular biology applications due to its high purity.
How do I prepare a 1M Tris Buffer solution?
Dissolve 121.14 grams of Tris Buffer in 800 ml of distilled water, adjust the pH with HCl, and bring the volume to 1 liter.
Is this product tested for endotoxins?
This grade is not specifically tested for endotoxins. For endotoxin-sensitive applications, consider an endotoxin-free grade.
Where can I buy authentic Tris Buffer online in India?
You can buy Tris Buffer Superior Extrapure AR, ACS, 99.9% online from eqipped, a trusted supplier of lab chemicals.