Specifications Table

Product Name – Tris EDTA buffer, for molecular biology, DNAse, RNAse, Protease free ready to use, pH 8.0
Quantity/Pack Size – 500ML
Form – Liquid (Ready-to-use solution)
Grade – Molecular Biology Grade (DNAse, RNAse, Protease-free)
Application – Nucleic acid stabilization, DNA/RNA extraction, PCR, and molecular cloning

Product Overview

This Tris EDTA buffer (pH 8.0) is a high-purity, ready-to-use solution designed for molecular biology applications where nuclease contamination is a critical concern. Formulated with ultra-pure Tris and EDTA, this buffer ensures optimal stability and protection of nucleic acids by chelating divalent metal ions, which can degrade DNA and RNA. The pH 8.0 formulation is ideal for maintaining the integrity of nucleic acids during extraction, purification, and downstream processes such as PCR, restriction digestion, and cloning. The buffer is rigorously tested to be free from DNase, RNase, and protease activity, ensuring reliable and reproducible results in sensitive molecular biology workflows. Its sterile, pre-mixed format eliminates the need for preparation, reducing the risk of contamination and saving valuable lab time. The solution is filtered through 0.22 µm membranes to guarantee sterility and is compatible with most standard molecular biology protocols. Whether used for plasmid preparation, genomic DNA isolation, or RNA protection, this buffer provides consistent performance and high-quality results, making it an essential reagent for research and diagnostic laboratories.

FAQs

1. What is the shelf life of this Tris EDTA buffer, and how should it be stored?

The buffer has a shelf life of 24 months from the date of manufacture when stored unopened at room temperature (15–25°C). Once opened, it should be tightly sealed and stored at 2–8°C to maintain stability and prevent microbial contamination. Avoid repeated freeze-thaw cycles, as they may alter the buffer’s pH and effectiveness.

2. Is this buffer compatible with downstream applications like PCR or sequencing?

Yes, this Tris EDTA buffer is fully compatible with downstream applications such as PCR, sequencing, and restriction enzyme digestion. Its nuclease-free and protease-free formulation ensures that nucleic acids remain intact and uncontaminated, which is critical for the accuracy of these techniques. The pH 8.0 is also optimal for most enzymatic reactions involved in molecular biology workflows.

3. Can this buffer be used for RNA stabilization, or is it specifically for DNA?

This buffer is suitable for both DNA and RNA stabilization. The EDTA in the formulation chelates metal ions that can catalyze RNA degradation, while the Tris component helps maintain a stable pH environment. However, for long-term RNA storage, it is recommended to use it in conjunction with RNA-specific stabilization reagents or store samples at -80°C to prevent degradation.

4. What is the difference between this buffer and a standard TE buffer?

This Tris EDTA buffer is a specialized, high-purity version of the standard TE buffer, with the key difference being its guaranteed freedom from DNase, RNase, and protease contaminants. Standard TE buffers may not undergo the same level of purification and testing, making them less suitable for sensitive applications where even trace amounts of nucleases can compromise results. Additionally, this buffer is pre-adjusted to pH 8.0 and is ready to use without further dilution or pH adjustment.

5. Does this buffer contain any additives or preservatives that could interfere with experiments?

No, this buffer is formulated without any additives, detergents, or preservatives that could interfere with molecular biology experiments. It consists solely of ultra-pure Tris and EDTA, ensuring compatibility with a wide range of applications. The absence of preservatives also makes it ideal for use in cell culture or other sensitive biological systems where chemical additives could introduce variability or toxicity.