Description
Specifications Table
| Product Name | Tris(Hydroxymethyl) Aminomethane Acetate for molecular biology (Tris Acetate Buffer), 99% |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Molecular Biology |
| Application | DNA/RNA electrophoresis, protein studies, buffer preparation |
Product Description
Tris Acetate Buffer is a critical reagent in molecular biology labs, widely used for DNA and RNA electrophoresis due to its stable pH buffering capacity. This high-purity (99%) grade ensures reliable results in gel electrophoresis, nucleic acid purification, and protein research. Whether you’re a student setting up your first gel or a researcher optimizing protocols, this buffer provides consistent performance.
At eqipped, we source this product from SRL Chem, a trusted name in laboratory chemicals, to guarantee quality and purity. The powder form allows for flexible preparation of buffer solutions tailored to your experimental needs. For those looking to buy Tris Acetate Buffer online, this product is an excellent choice for both academic and professional labs.
Need Tris Acetate Buffer for your next experiment? Order now and get lab-ready supplies delivered to your doorstep.
Tris Acetate Buffer is particularly valued for its role in maintaining the integrity of nucleic acids during electrophoresis. Its low ionic strength minimizes heat generation, making it ideal for high-resolution separations. Researchers often prefer it over other buffers for its compatibility with downstream applications like blotting and sequencing.
When preparing Tris Acetate EDTA (TAE) buffer, this product ensures optimal conditions for DNA fragmentation analysis. The 99% purity level reduces the risk of contaminants interfering with your results, which is crucial for sensitive applications like PCR and cloning.
FAQs
What is the shelf life of Tris Acetate Buffer powder?
When stored properly in a sealed container, the powder has a shelf life of 2-3 years. Prepared buffer solutions should be used within 6 months.
Can I use this buffer for protein electrophoresis?
Yes, Tris Acetate Buffer is suitable for protein electrophoresis, though Tris-Glycine is more commonly used for SDS-PAGE. Always check your protocol requirements.
How do I prepare 1X TAE buffer from this powder?
Dissolve 4.84 g of Tris Acetate and 1.14 mL of 0.5M EDTA (pH 8.0) in 1L of distilled water. Adjust pH to 8.3 if needed.
Is this buffer compatible with downstream applications like sequencing?
Yes, the high purity (99%) makes it suitable for sensitive applications including sequencing and cloning.
Where can I buy Tris Acetate Buffer online in India?
You can purchase authentic Tris Acetate Buffer from eqipped, with options for 100g and 500g packs to suit your lab needs.
