Description
Specifications Table
| Product Name | Tris(Hydroxymethyl)-Aminomethane Phosphate Dibasic Extrapure |
|---|---|
| Pack Size | 100 Gms |
| Form | Powder |
| Grade | Extrapure (98%) |
| Application | pH buffering in molecular biology, protein purification, and diagnostic assays |
Product Description
Tris Phosphate Dibasic is a high-purity biochemical reagent widely used in laboratories for its exceptional buffering capacity in the physiological pH range (7.0–9.0). This extrapure grade (98% purity) ensures minimal interference in sensitive applications, making it a preferred choice for researchers, students, and educators working in molecular biology, protein chemistry, and diagnostic testing.
Whether you’re preparing electrophoresis buffers, stabilizing enzymes, or conducting DNA/RNA experiments, this Tris Phosphate Dibasic delivers consistent performance. Its solubility in water and compatibility with common lab protocols make it a versatile addition to any research setup. For those looking to buy Tris Phosphate Dibasic online, eqipped offers a reliable supply with verified purity and fast shipping across India.
Need a dependable buffer for your experiments? Order Tris Phosphate Dibasic today and ensure accurate results every time.
Key Applications
This product is essential for:
- Preparing Tris-based buffers for gel electrophoresis (SDS-PAGE, agarose gels).
- Stabilizing proteins and nucleic acids during purification processes.
- Use in PCR, qPCR, and other amplification techniques where pH stability is critical.
- Diagnostic assays requiring precise pH control.
Why This Grade Matters
The extrapure (98%) grade of Tris Phosphate Dibasic is specifically designed to meet the demands of high-sensitivity applications. Unlike technical or industrial grades, this variant undergoes rigorous purification to remove trace metals, nucleases, and other contaminants that could compromise experimental integrity. For researchers and educators, this means fewer variables to account for and more reliable data.
Handling and Storage
Store the product in a cool, dry place away from direct sunlight. Keep the container tightly sealed to prevent moisture absorption, which can alter the product’s effectiveness. For long-term storage, consider desiccants to maintain stability. Always wear appropriate PPE (gloves, lab coat) when handling to avoid skin or eye contact.
Risk Note
Warning: Tris Phosphate Dibasic is a mild irritant. Avoid inhalation, ingestion, or contact with skin/eyes. In case of accidental exposure, rinse thoroughly with water and seek medical advice if irritation persists. Use in a well-ventilated area or under a fume hood when preparing solutions.
FAQs
What is the shelf life of Tris Phosphate Dibasic?
When stored properly in its original packaging, the product has a shelf life of 2–3 years. Always check for signs of degradation (e.g., clumping, discoloration) before use.
Can this product be used for cell culture applications?
Yes, but ensure it is dissolved in ultra-pure water and sterilized (e.g., filtration) before adding to cell culture media to avoid contamination.
How do I prepare a 1M Tris Phosphate buffer?
Dissolve 121.14 g of Tris Phosphate Dibasic in 800 mL of distilled water. Adjust the pH to the desired value using HCl or NaOH, then bring the volume to 1 L with water.
Is this product compatible with DNase/RNase-sensitive experiments?
Yes, the extrapure grade is tested for low nuclease activity, making it suitable for molecular biology applications involving nucleic acids.
Where can I buy Tris Phosphate Dibasic online in India?
You can purchase high-quality Tris Phosphate Dibasic from eqipped, a trusted supplier of lab chemicals with pan-India delivery and verified product authenticity.
